Abstract：
The design of single-atom nanozymes with dual active sites to increase their activity and for the detection and degradation of contaminants is rare and challenging. In this work, a single-atom nanozyme (FeCu-NC) based on a three-dimensional porous Fe/Cu dual active site was developed as a colorimetric sensor for both the quantitative analysis of isoniazid (INH) and the efficient degradation of levofloxacin (LEV). FeCu-NC was synthesized using a salt template and freeze-drying method with a three-dimensional hollow porous structure and dual active sites (Fe-Nx and Cu-Nx). In terms of morphology and structure, FeCu-NC exhibits excellent peroxidase-like activity and catalytic properties. Therefore, a colorimetric sensor was constructed around FeCu-NC for sensitive and rapid quantitative analysis of INH with a linear range of 0.9-10 μM and a detection limit as low as 0.3 μM, and the sensor was successfully applied to the analysis of INH in human urine. In addition, FeCu-NC promoted the efficient degradation of LEV by peroxymonosulfate activation, with a degradation rate of 90.4% for LEV at 30 min. This work sheds new light on the application of single-atom nanozymes to antibiotics for colorimetric sensing and degradation.
摘要：
设计具有双活性位点的单原子纳米酶以增加其活性并用于污染物的检测和降解是罕见且具有挑战性的。在这项工作中,开发了基于三维多孔Fe/Cu双活性位点的单原子纳米酶（FeCu-NC）作为比色传感器，用于定量分析异烟肼（INH）和高效降解左氧氟沙星（LEV）。FeCu-NC是使用盐模板和冷冻干燥方法合成的，具有三维中空多孔结构和双活性位点（Fe-Nx和Cu-Nx）。在形态和结构方面，FeCu-NC表现出优异的过氧化物酶样活性和催化性能。因此,在FeCu-NC周围构建了一个比色传感器，用于对异烟肼进行灵敏和快速的定量分析，线性范围为0.9-10μM，检出限低至0.3μM，该传感器已成功应用于人体尿液中异烟肼的分析。此外,FeCu-NC通过过氧单硫酸盐活化促进LEV的有效降解，30min时LEV的降解率为90.4%。这项工作为将单原子纳米酶应用于抗生素进行比色传感和降解提供了新的思路。
