Abstract：
OBJECTIVE: We investigated the presence of SARS-CoV-2 in free-ranging wildlife populations in Northeastern Minnesota on the Grand Portage Indian Reservation and Isle Royale National Park.
RESULTS: One hundred twenty nasal samples were collected from white-tailed deer, moose, grey wolves and black bears monitored for conservation efforts during 2022-2023. Samples were tested for viral RNA by RT-qPCR using the CDC N1/N2 primer set. Our data indicate that no wildlife samples were positive for SARS-CoV-2 RNA.
CONCLUSIONS: Continued surveillance is therefore crucial to better understand the changing landscape of zoonotic SARS-CoV-2 in the Upper Midwest.
RESULTS: One hundred twenty nasal samples were collected from white-tailed deer, moose, grey wolves and black bears monitored for conservation efforts during 2022-2023. Samples were tested for viral RNA by RT-qPCR using the CDC N1/N2 primer set. Our data indicate that no wildlife samples were positive for SARS-CoV-2 RNA.
CONCLUSIONS: Continued surveillance is therefore crucial to better understand the changing landscape of zoonotic SARS-CoV-2 in the Upper Midwest.
摘要：
目的：我们调查了明尼苏达州东北部大Portage印第安人保留区和皇家岛国家公园自由放养的野生动物种群中SARS-CoV-2的存在。
结果：收集了白尾鹿的120个鼻部样本，驼鹿,灰狼和黑熊在2022-2023年期间被监测保护工作。使用CDCN1/N2引物组通过RT-qPCR测试样品的病毒RNA。我们的数据表明，没有野生动物样本对SARS-CoV-2RNA呈阳性。
结论：因此，持续监测对于更好地了解中西部上地区人畜共患SARS-CoV-2的变化情况至关重要。
结果：收集了白尾鹿的120个鼻部样本，驼鹿,灰狼和黑熊在2022-2023年期间被监测保护工作。使用CDCN1/N2引物组通过RT-qPCR测试样品的病毒RNA。我们的数据表明，没有野生动物样本对SARS-CoV-2RNA呈阳性。
结论：因此，持续监测对于更好地了解中西部上地区人畜共患SARS-CoV-2的变化情况至关重要。
