Abstract：
OBJECTIVE: Diabetic nephropathy (DN) is one of the complications of diabetes mellitus (DM). This study aimed to investigate the association between genetic polymorphisms, specifically AGTR1 (rs5186) and TGF-β1 (rs1800470), and the risk of developing Diabetic nephropathy (DN) in type 2 diabetes mellitus patients, compared to those without DN and healthy controls.
METHODS: A case-control study was conducted on 165 diabetic patients (59 with diabetic nephropathy (DN) and 54 without DN (DM)), and 52 healthy controls (HC). The genotyping was done using amplification refractory mutation system method (ARMS-PCR). Age, gender, and duration of diabetes were matched across groups. Clinical parameters including FBS, RBS, HbA1C, creatinine, urea, SBP, DBP, total cholesterol, triglycerides, LDL, and BMI were assessed.
RESULTS: Diabetic patients with nephropathy exhibited significantly higher levels of clinical parameters compared to those without nephropathy and healthy controls. The risk allele of AGTR1 , C (p <0.0001), and risk allele containing genotypes AC (p <0.0001) and CC (p - 0.0010) were significantly higher in DN patients compared to DM and HC groups. Similarly, the TGF-β1 risk allele C (p - 0.0001), and corresponding genotypes TC (p - 0.0038) and CC (p - 0.0027) were significantly associated with increased risk of diabetic nephropathy compared to DM and HC groups.
CONCLUSIONS: The data showed significant association of AGTR1 (rs5186) and TGF-β1 (rs1800470) polymorphism with an increased risk of diabetic nephropathy in type 2 diabetes mellitus patients. More investigation will be required to disseminate the results, while increasing the samples size and using whole genome sequencing.
METHODS: A case-control study was conducted on 165 diabetic patients (59 with diabetic nephropathy (DN) and 54 without DN (DM)), and 52 healthy controls (HC). The genotyping was done using amplification refractory mutation system method (ARMS-PCR). Age, gender, and duration of diabetes were matched across groups. Clinical parameters including FBS, RBS, HbA1C, creatinine, urea, SBP, DBP, total cholesterol, triglycerides, LDL, and BMI were assessed.
RESULTS: Diabetic patients with nephropathy exhibited significantly higher levels of clinical parameters compared to those without nephropathy and healthy controls. The risk allele of AGTR1 , C (p <0.0001), and risk allele containing genotypes AC (p <0.0001) and CC (p - 0.0010) were significantly higher in DN patients compared to DM and HC groups. Similarly, the TGF-β1 risk allele C (p - 0.0001), and corresponding genotypes TC (p - 0.0038) and CC (p - 0.0027) were significantly associated with increased risk of diabetic nephropathy compared to DM and HC groups.
CONCLUSIONS: The data showed significant association of AGTR1 (rs5186) and TGF-β1 (rs1800470) polymorphism with an increased risk of diabetic nephropathy in type 2 diabetes mellitus patients. More investigation will be required to disseminate the results, while increasing the samples size and using whole genome sequencing.
摘要：
目的:糖尿病肾病(DN)是糖尿病(DM)的并发症之一。本研究旨在探讨基因多态性之间的关联，特别是AGTR1（rs5186）和TGF-β1（rs1800470），以及2型糖尿病患者发生糖尿病肾病（DN）的风险，与没有DN和健康对照者相比。
方法：对165例糖尿病患者（59例糖尿病肾病（DN）和54例非DN（DM））进行了病例对照研究，和52名健康对照（HC）。使用扩增难治性突变系统方法(ARMS-PCR)进行基因分型。年龄,性别,和糖尿病持续时间在各组之间进行匹配。临床参数包括FBS,苏格兰皇家银行,HbA1C,肌酐,尿素,SBP,DBP,总胆固醇,甘油三酯，LDL,和BMI进行了评估。
结果：患有肾病的糖尿病患者与没有肾病的患者和健康对照者相比，表现出明显更高水平的临床参数。AGTR1的风险等位基因，C（p<0.0001），与DM和HC组相比，DN患者中含有风险等位基因的基因型AC（p<0.0001）和CC（p-0.0010）明显更高。同样，TGF-β1风险等位基因C（p-0.0001），与DM和HC组相比，相应基因型TC（p-0.0038）和CC（p-0.0027）与糖尿病肾病风险增加显著相关。
结论：数据显示AGTR1(rs5186)和TGF-β1(rs1800470)多态性与2型糖尿病患者糖尿病肾病风险增加显著相关。将需要更多的调查来传播结果，同时增加样本量并使用全基因组测序。
方法：对165例糖尿病患者（59例糖尿病肾病（DN）和54例非DN（DM））进行了病例对照研究，和52名健康对照（HC）。使用扩增难治性突变系统方法(ARMS-PCR)进行基因分型。年龄,性别,和糖尿病持续时间在各组之间进行匹配。临床参数包括FBS,苏格兰皇家银行,HbA1C,肌酐,尿素,SBP,DBP,总胆固醇,甘油三酯，LDL,和BMI进行了评估。
结果：患有肾病的糖尿病患者与没有肾病的患者和健康对照者相比，表现出明显更高水平的临床参数。AGTR1的风险等位基因，C（p<0.0001），与DM和HC组相比，DN患者中含有风险等位基因的基因型AC（p<0.0001）和CC（p-0.0010）明显更高。同样，TGF-β1风险等位基因C（p-0.0001），与DM和HC组相比，相应基因型TC（p-0.0038）和CC（p-0.0027）与糖尿病肾病风险增加显著相关。
结论：数据显示AGTR1(rs5186)和TGF-β1(rs1800470)多态性与2型糖尿病患者糖尿病肾病风险增加显著相关。将需要更多的调查来传播结果，同时增加样本量并使用全基因组测序。
