PDF(Pubmed)
Abstract：
Gastropod molluscs such as Aplysia, Lymnaea, and Tritonia have been important for determining fundamental rules of motor control, learning, and memory because of their large, individually identifiable neurons. Yet only a small number of gastropod neurons have known molecular markers, limiting the ability to establish brain-wide structure-function relations. Here we combine high-throughput, single-cell RNA sequencing with in situ hybridization chain reaction in the nudibranch Berghia stephanieae to identify and visualize the expression of markers for cell types. Broad neuronal classes were characterized by genes associated with neurotransmitters, like acetylcholine, glutamate, serotonin, and GABA, as well as neuropeptides. These classes were subdivided by other genes including transcriptional regulators and unannotated genes. Marker genes expressed by neurons and glia formed discrete, previously unrecognized regions within and between ganglia. This study provides the foundation for understanding the fundamental cellular organization of gastropod nervous systems.
摘要：
腹足软体动物，如Aplysia，Lymnaea,和Tritonia对于确定运动控制的基本规则很重要，学习,和记忆，因为他们的大，可单独识别的神经元。然而，只有少数腹足纲神经元具有已知的分子标记，限制建立全脑结构-功能关系的能力。在这里，我们结合了高通量，单细胞RNA测序与原位杂交链式反应在裸枝Berghaneae中鉴定和可视化细胞类型标记物的表达。广泛的神经元类别的特征是与神经递质相关的基因，像乙酰胆碱，谷氨酸,血清素,还有GABA,以及神经肽。这些类别被其他基因细分，包括转录调节因子和未注释的基因。神经元和胶质细胞表达的标志基因形成离散，神经节内和神经节之间以前未被识别的区域。这项研究为理解腹足动物神经系统的基本细胞组织奠定了基础。
