Abstract：
Yellow fever (YF) is a disease caused by the homonymous flavivirus that can be prevented by a vaccine containing attenuated viruses. Since some individuals cannot receive this vaccine, the development of alternatives is desirable. Here, we developed a recombinant baculovirus (rBV) surface display platform utilizing a chimeric E-NS1 protein as a vaccine candidate. A pBacPAK9 vector containing the baculoviral GP64 signal peptide, the YFV prM, E, NS1 and the ectodomain of VSV-G sequences was synthesized. This transfer plasmid and the bAcGOZA bacmid were cotransfected into Sf9 cells, and an rBV-E-NS1 was obtained, which was characterized by PCR, WB, IFI and FACS analysis. Mice immunized with rBV-E-NS1 elicited a specific humoral and cellular immune response and were protected after YFV infection. In summary, we have developed an rBV that expresses YFV major antigen proteins on its surface, which opens new alternatives that can be tested in a mouse model.
摘要：
黄热病（YF）是由同源黄病毒引起的疾病，可以通过含有减毒病毒的疫苗来预防。因为有些人不能接种这种疫苗，开发替代品是可取的。这里,我们利用嵌合E-NS1蛋白作为疫苗候选物,开发了重组杆状病毒(rBV)表面展示平台.pBacPAK9载体含有杆状病毒GP64信号肽，YFVPRM，E,合成了NS1和VSV-G序列的胞外域。该转移质粒和bAcGOZA杆粒共转染到Sf9细胞中，获得了rBV-E-NS1，以PCR为特征，WB,FI和FACS分析。用rBV-E-NS1免疫的小鼠引起特异性体液和细胞免疫应答,并且在YFV感染后受到保护。总之,我们开发了一种在其表面表达YFV主要抗原蛋白的rBV，这打开了可以在小鼠模型中测试的新替代品。
