Abstract：
The development of a multifunctional wound dressing that can adapt to the shape of wounds and provide controlled drug release is crucial for diabetic patients. This study developed a carboxymethyl chitosan-based hydrogel dressing with enhanced mechanical properties and tissue adherence that were achieved by incorporating pectin (PE) and polydopamine (PDA) and loading the hydrogel with recombinant human epidermal growth factor (rhEGF). This EGF@PDA-CMCS-PE hydrogel demonstrated robust tissue adhesion, enhanced mechanical properties, and superior water retention and vapor permeability. It also exhibited significant antioxidant capacity. The results showed that EGF@PDA-CMCS-PE could effectively scavenge 2,2\'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate)， (1,1-diphenyl-2-picrylhydrazyl), and superoxide anions and increase superoxide dismutase and catalase levels in vivo. In vitro cytotoxicity and antibacterial assays showed good biocompatibility and antimicrobial properties. The sustained release of EGF by the hydrogel was confirmed, with a gradual release profile over 120 h. In vivo studies in diabetic mice showed that the hydrogel significantly accelerated wound healing, with a wound contraction rate of 97.84% by day 14. Histopathological analysis revealed that the hydrogel promoted fibroblast proliferation, neovascularization, and orderly connective tissue formation, leading to a more uniform and compact wound-healing process. Thus, EGF@PDA-CMCS-PE hydrogel presents a promising tool for managing chronic diabetic wounds, offering a valuable strategy for future clinical applications.
摘要：
开发能够适应伤口形状并提供受控药物释放的多功能伤口敷料对于糖尿病患者至关重要。这项研究开发了一种基于羧甲基壳聚糖的水凝胶敷料，该敷料具有增强的机械性能和组织粘附性，这是通过掺入果胶（PE）和聚多巴胺（PDA）并将水凝胶加载重组人表皮生长因子（rhEGF）来实现的。这种EGF@PDA-CMCS-PE水凝胶显示出强大的组织粘附力，增强的机械性能，和优越的保水性和蒸汽渗透性。它还表现出显著的抗氧化能力。结果表明，EGF@PDA-CMCS-PE可以有效清除2,2'-氮杂双-(3-乙基苯并噻唑啉-6-磺酸盐)，(1,1-二苯基-2-吡啶酰肼)，和超氧化物阴离子，并增加体内超氧化物歧化酶和过氧化氢酶的水平。体外细胞毒性和抗菌试验显示出良好的生物相容性和抗菌性能。证实了水凝胶对EGF的持续释放，在120小时内逐渐释放曲线。在糖尿病小鼠体内研究表明，水凝胶显着加速伤口愈合，到第14天，伤口收缩率为97.84%。组织病理学分析显示水凝胶促进成纤维细胞增殖,新生血管形成,和有序的结缔组织形成，导致更均匀和紧凑的伤口愈合过程。因此，EGF@PDA-CMCS-PE水凝胶是治疗慢性糖尿病伤口的一种有前途的工具，为未来的临床应用提供了有价值的策略。
