Abstract：
To explore a sustainable sophorolipid production, several hydrolysates from agricultural byproducts, such as wheat feed, rapeseed meal, coconut waste and palm waste were used as nitrogen sources. The four hydrolysates overperformed the controls after 168 h of fermentation using Starmerella bombicola ATCC 22214. Wheat feed and coconut waste hydrolysates were the most promising feedstocks presenting a linear relationship between yeast growth and diacetylated lactonic C18:1 production at total nitrogen concentrations below 1.5 g/L (R2 = 0.90 and 0.83, respectively). At 0.31 g/L total nitrogen, wheat feed hydrolysate achieved the highest production, yielding 72.20 ± 1.53 g/L of sophorolipid crude extract and 60.05 ± 0.56 g/L of diacetylated lactonic C18:1 at shake flask scale with productivities of 0.43 and 0.36 g/L/h, respectively. Results were confirmed in a 2-L bioreactor increasing 15 % diacetylated lactonic C18:1 production. Moreover, wheat feed hydrolysate supplemented only with a hydrophobic carbon source was able to produce mainly diacetylated lactonic C18:1 congener (88.5 % wt.), suggesting that the composition of the hydrolysate significantly influences the congeners profile. Overall, this study provides valuable insights into agricultural byproduct hydrolysates as potential nitrogen feedstocks for sophorolipid production and their further application on industrial biotechnology.
摘要：
探索一种可持续的槐糖脂生产，来自农业副产品的几种水解产物，如小麦饲料，菜籽粕,椰子废物和棕榈废物被用作氮源。在使用StarmerelabbombicolaATCC22214发酵168小时后，四种水解产物的表现优于对照。小麦饲料和椰子废物水解产物是最有前途的原料，在总氮浓度低于1.5g/L（R2分别为0.90和0.83）时，酵母生长与二乙酰化内酯C18：1产量之间呈线性关系。在0.31g/L的总氮，小麦饲料水解物产量最高，在摇瓶规模下产生72.20±1.53g/L的槐糖脂粗提物和60.05±0.56g/L的二乙酰化内酯C18：1，生产率为0.43和0.36g/L/h，分别。结果在2-L生物反应器中得到证实,提高15%的二乙酰化内酯C18:1产量。此外，仅补充疏水性碳源的小麦饲料水解物能够主要产生二乙酰化的内酯C18：1同源物（88.5wt％。),这表明水解产物的组成显着影响同源物的概况。总的来说,这项研究提供了有价值的见解，农业副产品水解产物作为潜在的氮原料，用于生产槐糖脂，并进一步应用于工业生物技术。
