Abstract：
The effects of antibiotics, such as tetracycline, sulfamethoxazole, and ciprofloxacin, on functional microorganisms are of significant concern in wastewater treatment. This study observed that Acinetobacter indicus CZH-5 has a limited capacity to remove nitrogen and phosphorus using antibiotics (5 mg/L) as the sole carbon source. When sodium acetate was supplied (carbon/nitrogen ratio = 7), the average removal efficiencies of ammonia-N, total nitrogen, and orthophosphate-P increased to 52.46 %, 51.95 %, and 92.43 %, respectively. The average removal efficiencies of antibiotics were 84.85 % for tetracycline, 39.32 % for sulfamethoxazole, 18.85 % for ciprofloxacin, and 23.24 % for their mixtures. Increasing the carbon/nitrogen ratio to 20 further improved the average removal efficiencies to 72.61 % for total nitrogen and 97.62 % for orthophosphate-P (5 mg/L antibiotics). Additionally, the growth rate and pollutant removal by CZH-5 were unaffected by the presence of 0.1-1 mg/L antibiotics. Transcriptomic analysis revealed that the promoted translation of aceE, aarA, and gltA genes provided ATP and proton -motive forces. The nitrogen metabolism and polyphosphate genes were also affected. The expression of acetate kinase, dehydrogenase, flavin mononucleotide enzymes, and cytochrome P450 contributed to antibiotic degradation. Intermediate metabolites were investigated to determine the reaction pathways.
摘要：
抗生素的作用，比如四环素,磺胺甲恶唑,还有环丙沙星,功能微生物在废水处理中受到关注。这项研究观察到，不动杆菌CZH-5使用抗生素（5mg/L）作为唯一的碳源去除氮和磷的能力有限。当供应乙酸钠时（碳/氮比=7），氨氮的平均去除效率，总氮,正磷酸盐-P增加到52.46%，51.95%,92.43%，分别。抗生素对四环素的平均去除率为84.85%,磺胺甲恶唑为39.32%，环丙沙星的18.85%，和23.24%的混合物。将碳/氮比提高到20，将总氮的平均去除率进一步提高到72.61％，将正磷酸盐P（5mg/L抗生素）的平均去除率提高到97.62％。此外,CZH-5的生长速率和污染物去除不受0.1-1mg/L抗生素的影响。转录组学分析显示，aceE的促进翻译，aarA,gltA基因提供ATP和质子动力。氮代谢和多磷酸盐基因也受到影响。乙酸激酶的表达，脱氢酶，黄素单核苷酸酶，细胞色素P450有助于抗生素降解。研究中间代谢物以确定反应途径。
