Abstract：
Various adjuvants have been tested clinically for patients with problems with embryo implantation during in vitro fertilization (IVF)-embryo transfer (ET). Vitamin D3, an essential modulator of various physiological processes, has received attention as an important adjuvant for successful pregnancy, as many studies have shown a strong association between vitamin D deficiency and implantation failure and fetal growth restriction. However, vitamin D has been widely utilized in different protocols, resulting in non-reproducible and debatable outcomes. In the present study, we demonstrated that cyclic intrauterine administration of vitamin D3 increased endometrial receptivity and angiogenesis, which could be attributed to increased recruitment of uterus-resident natural killer cells. In particular, cyclic treatment of vitamin D3 promoted stable attachment of the embryo onto endometrial cells in vitro, suggesting its merit during the early stage of embryo implantation to support the initial maternal-fetal interactions. Our findings suggest that women with repeated implantation failure may benefit from the use of vitamin D3 as a risk-free adjuvant prior to IVF-ET procedures to improve the uterine environment, and make it favorable for embryo implantation.
摘要：
已经针对在体外受精(IVF)-胚胎移植(ET)期间存在胚胎植入问题的患者临床测试了各种佐剂。维生素D3是各种生理过程的重要调节剂，作为成功怀孕的重要辅助手段受到了关注，因为许多研究表明维生素D缺乏与着床失败和胎儿生长受限之间有很强的关联.然而,维生素D已被广泛用于不同的方案，导致不可重复和有争议的结果。在本研究中,我们证明，循环宫内施用维生素D3增加子宫内膜容受性和血管生成，这可能归因于子宫内自然杀伤细胞的募集增加。特别是,维生素D3的循环处理促进了胚胎在体外子宫内膜细胞上的稳定附着，提示其在胚胎植入早期阶段的优点，以支持最初的母胎相互作用。我们的研究结果表明，反复植入失败的女性可能受益于在IVF-ET手术前使用维生素D3作为无风险佐剂，以改善子宫环境。并使其有利于胚胎植入。
