PDF(Pubmed)
Abstract：
Mycosis fungoides (MF) is the most common primary cutaneous T-cell lymphoma (CTCL) with its etiology not yet fully understood. Interleukin (IL)-35 is an inhibitory cytokine that belongs to the IL-12 family. Elevated IL-35 in the plasma and the tumor microenvironment increases tumorigenesis and indicates poor prognosis in different types of malignancies. The objective of this study is to estimate the expression levels of IL-35 in tissue and serum of MF patients versus healthy controls. This case-control study included 35 patients with patch, plaque, and tumor MF as well as 30 healthy controls. Patients were fully assessed, and serum samples and lesional skin biopsies were taken prior to starting treatment. The IL-35 levels were measured in both serum and tissue biopsies by ELISA technique. Both tissue and serum IL-35 levels were significantly higher in MF patients than in controls (P < 0.001) and tissue IL-35 was significantly higher than serum IL-35 in MF patients (P < 0.001). Tissue IL-35 was significantly higher in female patients and patients with recurrent MF compared to male patients and those without recurrent disease (P < 0.001). Since both tissue and serum IL-35 levels are increased in MF, IL-35 is suggested to have a possible role in MF pathogenesis. IL-35 can be a useful diagnostic marker for MF. Tissue IL-35 can also be an indicator of disease recurrence.
摘要：
真菌病（MF）是最常见的原发性皮肤T细胞淋巴瘤（CTCL），其病因尚未完全了解。白细胞介素(IL)-35是属于IL-12家族的抑制性细胞因子。血浆和肿瘤微环境中IL-35的升高会增加肿瘤发生，并表明不同类型恶性肿瘤的预后不良。这项研究的目的是评估MF患者与健康对照的组织和血清中IL-35的表达水平。这项病例对照研究包括35例患者，牌匾,和肿瘤MF以及30名健康对照。对患者进行全面评估，在开始治疗前采集血清样本和皮损活检。通过ELISA技术测量血清和组织活检中的IL-35水平。MF患者的组织和血清IL-35水平均明显高于对照组（P<0.001），MF患者的组织IL-35明显高于血清IL-35（P<0.001）。与男性患者和无复发性疾病患者相比，女性患者和复发性MF患者的组织IL-35明显更高（P<0.001）。由于MF中组织和血清IL-35水平均增加，IL-35可能在MF的发病机制中起作用。IL-35可以是MF的有用诊断标记。组织IL-35也可以是疾病复发的指标。
