Abstract：
The genus Sarcocystis includes protozoan parasites with an indirect life cycle. Sarcocystis spp. can infect various animal species and humans, causing sarcocystosis, a parasitosis of economic importance and zoonotic concern. Wild boars can act as intermediate hosts for Sarcocystis miescheriana and the zoonotic Sarcocystis suihominis that infects humans by consumption of raw or undercooked infected swine meat. In the present study, the diaphragmatic muscle tissue of 123 wild boars hunted in Greece was examined to determine the frequency of Sarcocystis spp. The samples were examined by tissue compression and molecular techniques. Under light microscopy, 34 out of 123 (27.6%) wild boars tested positive for Sarcocystis spp., while a higher infection prevalence (75%) was revealed by multiplex PCR performed in 100 of the samples. The partial mtDNA cox1 gene (~ 1100 bp) of 20 samples tested positive for S. miescheriana by multiplex PCR was amplified and sequenced. Sarcocystis miescheriana was identified as the only species involved in these infections. This is the first study on the prevalence of Sarcocystis spp. in wild animals in Greece. Further, large-scale surveys are needed to assess the prevalence and species of this parasite in Greece and to design efficient control and preventive measures in a One Health perspective.
摘要：
肉囊虫属包括具有间接生命周期的原生动物寄生虫。结节虫。可以感染各种动物和人类，导致肌囊炎,具有经济重要性和人畜共患关注的寄生虫病。野猪可以作为肉孢子虫和人畜共患的猪孢子虫的中间宿主，它们通过食用生的或未煮熟的感染猪肉来感染人类。在本研究中,检查了在希腊猎杀的123头野猪的the肌组织，以确定肉孢子虫的频率。通过组织压缩和分子技术检查样品。在光学显微镜下，123只野猪中有34只（27.6％）的肉囊虫属试验呈阳性。,而在100个样本中进行的多重PCR显示出更高的感染率（75％）。通过多重PCR扩增并测序了20个样品的mtDNAcox1基因（〜1100bp）。被确定为与这些感染有关的唯一物种。这是有关结节虫的患病率的第一项研究。在希腊的野生动物。Further,需要进行大规模调查，以评估希腊这种寄生虫的患病率和种类，并从“一个健康”的角度设计有效的控制和预防措施。
