PDF(Pubmed)
Abstract：
The transition towards a sustainable bioeconomy requires the development of highly efficient bioprocesses that enable the production of bulk materials at a competitive price. This is particularly crucial for driving the commercialization of polyhydroxyalkanoates (PHAs) as biobased and biodegradable plastic substitutes. Among these, the copolymer poly(hydroxybutyrate-co-hydroxyhexanoate) (P(HB-co-HHx)) shows excellent material properties that can be tuned by regulating its monomer composition. In this study, we developed a high-cell-density fed-batch strategy using mixtures of fructose and canola oil to modulate the molar composition of P(HB-co-HHx) produced by Ralstonia eutropha Re2058/pCB113 at 1-L laboratory scale up to 150-L pilot scale. With cell densities >100 g L-1 containing 70-80 wt% of PHA with tunable HHx contents in the range of 9.0-14.6 mol% and productivities of up to 1.5 g L-1 h-1, we demonstrate the tailor-made production of P(HB-co-HHx) at an industrially relevant scale. Ultimately, this strategy enables the production of PHA bioplastics with defined material properties on the kilogram scale, which is often required for testing and adapting manufacturing processes to target diverse applications.
摘要：
向可持续生物经济的过渡需要开发高效的生物工艺，以具有竞争力的价格生产散装材料。这对于推动聚羟基链烷酸酯（PHA）作为生物基和可生物降解的塑料替代品的商业化尤为重要。其中,共聚物聚(羟基丁酸酯-共-羟基己酸酯)(P(HB-共-HHx))显示出优异的材料性质,其可以通过调节其单体组成来调节。在这项研究中,我们使用果糖和菜籽油的混合物开发了一种高细胞密度的分批补料策略，以调节1-L实验室规模至150-L中试规模的RalstoniaeutrophaRe2058/pCB113产生的P（HB-co-HHx）的摩尔组成。细胞密度>100gL-1，含有70-80wt％的PHA，可调节的HHx含量在9.0-14.6mol％的范围内，生产率高达1.5gL-1h-1，我们证明了定制生产在工业相关规模上生产P（HB-co-HHx）。最终,这种策略使得PHA生物塑料的生产能够在千克规模上具有确定的材料特性，这通常是测试和调整制造工艺以针对不同应用所需的。
