Abstract：
The mechanism of bioluminescence in the luminous land snails remains largely unknown. Here, we analyzed basic biochemical properties of the luminous land snail, Quantula weinkauffiana. The luminescence activity was extracted from the light organ located near the mouth using a neutral buffer containing detergent. The reaction of the crude buffer extract was triggered by the addition of only hydrogen peroxide (H2O2). These results are inconsistent with the single precedent report on the bioluminescence in the Quantula striata by Shimomura and Haneda in 1986, in which the luminescence of the buffer extract (without detergent) from the light organ was induced by the coaddition of three indispensable components H2O2, ferrous ion, and 2-mercaptoethanol. Based on the present findings, we suggested that an insoluble photoprotein is involved in the bioluminescence of the luminous land snails and the luminescence reaction is simply triggered by H2O2.
摘要：
发光的陆地蜗牛中的生物发光机制仍然未知。这里,我们分析了发光的土地蜗牛的基本生化特性，Quantulaweinkauffiana.使用含有去污剂的中性缓冲液从位于口腔附近的光器官中提取发光活性。通过仅添加过氧化氢(H2O2)来触发粗缓冲液提取物的反应。这些结果与Shimomura和Haneda在1986年关于Quantulastriata中生物发光的单一先例报告不一致，在该报告中，来自光器官的缓冲提取物（不含洗涤剂）的发光是由三种必不可少的组分H2O2，亚铁离子，和2-巯基乙醇。根据目前的调查结果，我们建议不溶性光蛋白参与发光的陆地蜗牛的生物发光，并且发光反应仅由H2O2触发。
