PDF(Pubmed)
Abstract：
BACKGROUND: The analysis of differentially expressed genes in muscle tissues of sheep at different ages is helpful to analyze the gene expression trends during muscle development. In this study, the longissimus dorsi muscle of pure breeding Hu sheep (H), Suffolk sheep and Hu sheep hybrid F1 generation (SH) and East Friesian and Hu sheep hybrid sheep (EHH) three strains of sheep born 2 days (B2) and 8 months (M8) was used as the research object, and transcriptome sequencing technology was used to identify the differentially expressed genes of sheep longissimus dorsi muscle in these two stages. Subsequently, GO and KEGG enrichment analysis were performed on the differential genes. Nine differentially expressed genes were randomly selected and their expression levels were verified by qRT-PCR.
RESULTS: The results showed that 842, 1301 and 1137 differentially expressed genes were identified in H group, SH group and EHH group, respectively. Among them, 191 differential genes were enriched in these three strains, including pre-folding protein subunit 6 (PFDN6), DnaJ heat shock protein family member A4 (DNAJA4), myosin heavy chain 8 (MYH8) and so on. GO and KEGG enrichment analysis was performed on 191 differentially expressed genes shared by the three strains to determine common biological pathways. The results showed that the differentially expressed genes were significantly enriched in ribosomes, unfolded protein binding, FoxO signaling pathway, glycolysis / glycogen generation and glutathione signaling pathway that regulate muscle protein synthesis and energy metabolism. The results of qRT-PCR were consistent with transcriptome sequencing, which proved that the sequencing results were reliable.
CONCLUSIONS: Overall, this study revealed the important genes and signaling pathways related to sheep skeletal muscle development, and the result laid a foundation for further understanding the mechanism of sheep skeletal muscle development.
RESULTS: The results showed that 842, 1301 and 1137 differentially expressed genes were identified in H group, SH group and EHH group, respectively. Among them, 191 differential genes were enriched in these three strains, including pre-folding protein subunit 6 (PFDN6), DnaJ heat shock protein family member A4 (DNAJA4), myosin heavy chain 8 (MYH8) and so on. GO and KEGG enrichment analysis was performed on 191 differentially expressed genes shared by the three strains to determine common biological pathways. The results showed that the differentially expressed genes were significantly enriched in ribosomes, unfolded protein binding, FoxO signaling pathway, glycolysis / glycogen generation and glutathione signaling pathway that regulate muscle protein synthesis and energy metabolism. The results of qRT-PCR were consistent with transcriptome sequencing, which proved that the sequencing results were reliable.
CONCLUSIONS: Overall, this study revealed the important genes and signaling pathways related to sheep skeletal muscle development, and the result laid a foundation for further understanding the mechanism of sheep skeletal muscle development.
摘要：
背景：分析不同年龄绵羊肌肉组织中的差异表达基因有助于分析肌肉发育过程中的基因表达趋势。在这项研究中,纯种湖羊（H）的背肌，以萨福克羊和湖羊杂交F1代(SH)和东弗利西亚羊和湖羊杂交绵羊(EHH)3株出生2天(B2)和8个月(M8)的绵羊为研讨对象，利用转录组测序技术鉴定了绵羊背最长肌在这两个阶段的差异表达基因。随后，对差异基因进行GO和KEGG富集分析。随机选择9个差异表达基因，并通过qRT-PCR验证其表达水平。
结果：结果显示，在H组中鉴定出842、1301和1137个差异表达基因，SH组和EHH组,分别。其中,在这三个菌株中富集了191个差异基因，包括预折叠蛋白亚基6（PFDN6），DnaJ热休克蛋白家族成员A4(DNAJA4),肌球蛋白重链8(MYH8)等。对三个菌株共有的191个差异表达基因进行GO和KEGG富集分析，以确定共同的生物学途径。结果表明,差异表达基因在核糖体中显著富集,未折叠的蛋白质结合，FoxO信号通路,糖酵解/糖原生成和谷胱甘肽信号通路调节肌肉蛋白合成和能量代谢。qRT-PCR检测结果与转录组测序结果一致,证明测序结果可靠。
结论：总体而言，本研究揭示了与绵羊骨骼肌发育相关的重要基因和信号通路,为进一步了解绵羊骨骼肌发育机制奠定了基础。
结果：结果显示，在H组中鉴定出842、1301和1137个差异表达基因，SH组和EHH组,分别。其中,在这三个菌株中富集了191个差异基因，包括预折叠蛋白亚基6（PFDN6），DnaJ热休克蛋白家族成员A4(DNAJA4),肌球蛋白重链8(MYH8)等。对三个菌株共有的191个差异表达基因进行GO和KEGG富集分析，以确定共同的生物学途径。结果表明,差异表达基因在核糖体中显著富集,未折叠的蛋白质结合，FoxO信号通路,糖酵解/糖原生成和谷胱甘肽信号通路调节肌肉蛋白合成和能量代谢。qRT-PCR检测结果与转录组测序结果一致,证明测序结果可靠。
结论：总体而言，本研究揭示了与绵羊骨骼肌发育相关的重要基因和信号通路,为进一步了解绵羊骨骼肌发育机制奠定了基础。
