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Abstract：
Blood clot formation, a crucial process in hemostasis and thrombosis, has garnered substantial attention for its implications in various medical conditions. Microscopic examination of blood clots provides vital insights into their composition and structure, aiding in the understanding of clot pathophysiology and the development of targeted therapeutic strategies. This study explores the use of topological data analysis (TDA) to assess plasma clot characteristics microscopically, focusing on the identification of the elements components, holes and Wasserstein distances. This approach should enable researchers to objectively classify fibrin networks based on their topologic architecture. We tested this mathematical characterization approach on plasma clots formed in static conditions from porcine and human citrated plasma samples, where the effect of dilution and direct thrombin inhibition was explored. Confocal microscopy images showing fluorescence labeled fibrin networks were analyzed. Both treatments resulted in visual differences in plasma clot architecture, which could be quantified using TDA. Significant differences between baseline and diluted samples, as well as blood anticoagulated with argatroban, were detected mathematically. Therefore, TDA could be indicative of clots with compromised stability, providing a valuable tool for thrombosis risk assessment. In conclusion, microscopic examination of plasma clots, coupled with Topological Data Analysis, offers a promising avenue for comprehensive characterization of clot microstructure. This method could contribute to a deeper understanding of clot pathophysiology and thereby refine our ability to assess clot characteristics.
摘要：
血块形成,止血和血栓形成的关键过程，它在各种医疗条件下的影响引起了广泛的关注。血凝块的显微镜检查提供了对其组成和结构的重要见解，帮助了解血凝块病理生理学和制定有针对性的治疗策略。这项研究探讨了使用拓扑数据分析（TDA）来评估血浆凝块的微观特征，专注于元素组件的识别，洞和Wasserstein距离。这种方法应该使研究人员能够根据其拓扑结构对纤维蛋白网络进行客观分类。我们在静态条件下从猪和人柠檬酸化血浆样品中形成的血浆凝块上测试了这种数学表征方法，其中研究了稀释和直接凝血酶抑制的效果。分析显示荧光标记的纤维蛋白网络的共聚焦显微镜图像。两种治疗都导致血浆凝块结构的视觉差异，这可以用TDA量化。基线和稀释样品之间的显着差异，以及用阿加曲班抗凝血的血液，被数学检测到。因此,TDA可能指示稳定性受损的凝块，为血栓形成风险评估提供了有价值的工具。总之,血浆凝块的显微镜检查，再加上拓扑数据分析，为全面表征凝块微观结构提供了一条有前途的途径。这种方法可以有助于更深入地了解凝块病理生理学，从而完善我们评估凝块特征的能力。
