Abstract：
Biogenic coalbed methane (CBM) is a developing clean energy source. However, it is unclear how the mechanisms of bio-methane production with different sizes of coal. In this work, pulverized coal (PC) and lump coal (LC) were used for methane production by mixed fungi-methanogen microflora. The lower methane production from LC was observed. The aromatic carbon of coal was degraded slightly by 2.17% in LC, while 11.28% in PC. It is attributed to the proportion of lignin-degrading fungi, especially Penicillium, which was reached 67.57% in PC on the 7th day, higher than that of 11.38% in LC. The results suggested that the limited interaction area in LC led to microorganisms hardly utilize aromatics. It also led the accumulation of aromatic organics in the fermentation broth in PC. Increasing the reaction area of coal and facilitating the conversion of aromatic carbon are suggested means to increase methane production in situ.
摘要：
生物煤层气（CBM）是一种新兴的清洁能源。然而,目前尚不清楚如何使用不同大小的煤生产生物甲烷的机制。在这项工作中,煤粉（PC）和块煤（LC）用于混合真菌-产甲烷菌微生物区系的甲烷生产。观察到来自LC的较低的甲烷产量。煤中的芳香碳在LC中略微降解了2.17%，而PC占11.28%。这归因于木质素降解真菌的比例，尤其是青霉菌,在第7天，PC达到67.57%，高于LC的11.38%。结果表明，块煤中有限的相互作用面积导致微生物几乎不利用芳烃。它还导致了PC发酵液中芳香有机物的积累。建议增加煤的反应面积并促进芳族碳的转化，以增加原位甲烷产量。
