Abstract：
Local anaesthetics (LAs) can have detrimental effects on rat, bovine, canine, and human tendon tissues and cells. Currently, there has been no available data on the impact of these drugs on equine tenocytes. Even if LA injection for managing painful tendon conditions in horses is limited, it is usually used via intra-articular, intrasynovial, perineural, and intrathecal as well as for lameness examinations. In this in vitro study, the cytotoxic effects of LAs, including lidocaine, mepivacaine, and bupivacaine on equine tenocytes, in the presence and absence of platelet rich plasma (PRP), were investigated. PRP accelerates tissue healing and can exert cytoprotective effects on different cell types exposed to different stressful conditions, including drugs. Results indicated that the exposure to LAs significantly reduced tenocytes viability in dose- and time-dependent manners while PRP was able to counteract their cytotoxic effects. Furthermore, microscopy and flow cytometry analyses revealed apoptosis and necrosis in equine tenocytes exposed to these drugs, that were both reduced when PRP was in the medium. These findings highlight the importance of considering the tenocyte toxicity associated with intrathecal and intraneural LA injections, as they might affect tenocytes or reduce the efficacy of associated therapies. Moreover, this study also highlights the protective effects of PRP, which could make LA injections safer.
摘要：
局部麻醉剂（LA）可能对大鼠产生有害影响，牛,犬和人类肌腱组织和细胞。目前,目前尚无关于这些药物对马肌腱细胞影响的可用数据.即使用于管理马疼痛肌腱状况的LA注射是有限的，它通常通过关节内使用，滑膜内,神经周,鞘内以及跛行检查。在这项体外研究中，LA的细胞毒性作用，包括利多卡因,甲哌卡因,和布比卡因对马肌腱细胞的作用，在存在和不存在富血小板血浆（PRP）的情况下，被调查了。PRP可加速组织愈合，并对暴露于不同应激条件的不同细胞类型发挥细胞保护作用，包括毒品。结果表明，暴露于LA以剂量和时间依赖性方式显着降低了肌腱细胞的活力，而PRP能够抵消其细胞毒性作用。此外，显微镜和流式细胞术分析显示暴露于这些药物的马肌腱细胞凋亡和坏死，当PRP在培养基中时，两者都减少了。这些发现强调了考虑鞘内和耳内注射LA相关的肌腱细胞毒性的重要性。因为它们可能会影响肌腱细胞或降低相关疗法的疗效。此外，这项研究还强调了PRP的保护作用，这可以让洛杉矶注射更安全。
