Abstract：
Lymphatic transport shapes the homeostatic immune repertoire of lymph nodes (LNs). LN-resident memory T cells (TRMs) play an important role in site-specific immune memory, yet how LN TRMs form de novo after viral infection remains unclear. Here, we tracked the anatomical distribution of antiviral CD8+ T cells as they seeded skin and LN TRMs using a model of vaccinia virus-induced skin infection. LN TRMs localized to the draining LNs (dLNs) of infected skin, and their formation depended on the lymphatic egress of effector CD8+ T cells from the skin, already poised for residence. Effector CD8+ T cell transit through skin was required to populate LN TRMs in dLNs, a process reinforced by antigen encounter in skin. Furthermore, LN TRMs were protective against viral rechallenge in the absence of circulating memory T cells. These data suggest that a subset of tissue-infiltrating CD8+ T cells egress from tissues during viral clearance and establish a layer of regional protection in the dLN basin.
摘要：
淋巴运输塑造了淋巴结（LN）的稳态免疫库。LN-常驻记忆T细胞(TRMs)在位点特异性免疫记忆中起重要作用,然而,LNTRMs如何在病毒感染后从头形成尚不清楚.这里,我们使用痘苗病毒诱导的皮肤感染模型追踪了抗病毒CD8+T细胞接种皮肤和LNTRM时的解剖分布.LNTRM定位在受感染皮肤的引流LN（dLN），它们的形成取决于效应CD8+T细胞从皮肤的淋巴流出，已经准备好居住。效应CD8+T细胞通过皮肤转运需要在dLN中填充LNTRM，皮肤中的抗原强化的过程。此外，LNTRM在不存在循环记忆T细胞的情况下对病毒再攻击具有保护性。这些数据表明，在病毒清除过程中，组织浸润的CD8T细胞亚群从组织中流出，并在dLN盆地中建立了一层区域保护。
