Abstract：
The aim of this study was to analyze the role of thiol/disulfide homeostasis (TDH) parameters as an indicator of oxidative stress in acute appendicitis (AA). PubMed, EMBASE, Web of Science, and Scopus databases were systematically searched. Studies reporting on TDH in AA (both complicated and uncomplicated cases) were included. The comparator group were healthy controls. The TDH domain was compared between the groups using anti-oxidant parameters, namely native thiol and total thiol levels, and native thiol/total thiol ratio; and oxidant parameters, namely disulfide level, disulfide/native thiol ratio, and disulfide/total thiol ratio. The statistical analysis was performed using a random-effects model. The methodological quality of the studies was assessed utilizing the Newcastle-Ottawa scale. Eleven studies with a total of 926 subjects, comprising 457 patients with uncomplicated appendicitis, 147 with complicated appendicitis, and 322 healthy controls were included. Our study demonstrated significantly increased oxidative stress in AA as compared to healthy controls in all TDH parameters and significantly lower total thiol levels in complicated AA as compared to uncomplicated AA. Due to a poor methodological quality in five out of eleven studies, future prospective studies with adequate power are essential to validate these observations and refine the diagnostic approaches to AA.
摘要：
这项研究的目的是分析硫醇/二硫化物稳态（TDH）参数作为急性阑尾炎（AA）中氧化应激指标的作用。PubMed,EMBASE,WebofScience,和Scopus数据库进行了系统搜索。包括AA中TDH的研究报告（包括复杂和不复杂的病例）。比较组为健康对照。使用抗氧化剂参数比较各组之间的TDH域，即天然硫醇和总硫醇水平，和天然硫醇/总硫醇比；和氧化剂参数，即二硫化物水平，二硫化物/天然硫醇比，和二硫化物/总硫醇比。使用随机效应模型进行统计分析。使用纽卡斯尔-渥太华量表评估研究的方法学质量。11项研究共926名受试者，包括457名无并发症阑尾炎患者，147患有复杂的阑尾炎，和322名健康对照被包括在内。我们的研究表明，在所有TDH参数中，与健康对照相比，AA的氧化应激显着增加，并且与不复杂的AA相比，复杂的AA的总硫醇水平显着降低。由于11项研究中有5项的方法学质量较差，未来具有足够功效的前瞻性研究对于验证这些观察结果和完善AA的诊断方法至关重要.
