Complete genome sequence of a Pseudomonas fluorescens bacteriophage UNO-G1W1 isolated from freshwater ice in Nebraska.

从内布拉斯加州淡水冰中分离出的荧光假单胞菌噬菌体 UNO - G1W1 的完整基因组序列。

• 发表时间: 2024 Jul 18
• 来源期刊: Microbiol Resour Announc
• DOI: 10.1128/mra.00384-24
• PMID: 38847506
• 文章类型: Journal Article
• 作者列表: Schulze TT,Neville AJ,Watson GF,Sanford AG,Won HI,Conrin ME,Eastman CG,Lui LM,Alizai MY,Walters MJ,Davis PH,Tapprich WE

关键词: DTR; Oxford Nanopore; Pseudomonas; Pseudomonas fluorescens; bacteriophages; genomes; genomics; hybrid assembly; long-read seqeuncing

Abstract

We provide the complete genome sequence for a novel Pseudomonas fluorescens bacteriophage named UNO-G1W1. This phage was isolated from a single ice cover sampling. The genome was sequenced on the Nanopore MinION, generated with the direct terminal repeat-phage-pipeline and polished with Illumina short reads. Sequence identity classifies the phage as an otagovirus.

摘要

我们提供了一种名为UNO-G1W1的新型荧光假单胞菌噬菌体的完整基因组序列。从单个冰盖取样中分离该噬菌体。基因组在纳米孔Minion上测序，用直接末端重复噬菌体管道产生，并用Illumina短读数抛光。序列同一性将噬菌体分类为耳病毒。