Abstract：
OBJECTIVE: To date, few studies have considered the influence of psychological factors on chronic prostatitis (PRO) models. Here, we aimed to refine a murine PRO model combining chemically induced prostatitis with psychological stress.
METHODS: A total of 40 mice were randomly divided into four groups: normal control (NC) group, PRO group, water avoidance stress (WAS) group, and PRO + WAS group. Ten mice were assigned to each group: five for cystometrograms (CMGs) and five for von Frey testing and histological analysis. PRO was induced through a prostatic injection of 10% paraformaldehyde. The WAS mice were placed on the middle platform for 1 h per day for 10 consecutive days.
RESULTS: The results of the von Frey test demonstrated that both WAS and PRO induced bladder hyperalgesia in mice, and the WAS + PRO group showed significant pelvic pain symptoms either. The CMG results suggested that the PRO group, the WAS group, and the PRO + WAS group all exhibited bladder overactivity, presented as a shortened micturition interval and decreased threshold pressure evoking bladder contraction. The symptoms of the PRO group and the PRO + WAS group were more severe than those of the WAS group. The tissue staining results indicated that WAS itself caused only mild prostatic inflammation but could significantly aggravate chemical-induced prostatic inflammation, as well as the total number of mast cells and proportion of activated mast cells.
CONCLUSIONS: Our refined murine PRO model could manifest persistent bladder overactivity, pelvic hyperalgesia and prostatic inflammation. WAS could induce mild prostatic inflammation and aggravate primary prostatic inflammation.
METHODS: A total of 40 mice were randomly divided into four groups: normal control (NC) group, PRO group, water avoidance stress (WAS) group, and PRO + WAS group. Ten mice were assigned to each group: five for cystometrograms (CMGs) and five for von Frey testing and histological analysis. PRO was induced through a prostatic injection of 10% paraformaldehyde. The WAS mice were placed on the middle platform for 1 h per day for 10 consecutive days.
RESULTS: The results of the von Frey test demonstrated that both WAS and PRO induced bladder hyperalgesia in mice, and the WAS + PRO group showed significant pelvic pain symptoms either. The CMG results suggested that the PRO group, the WAS group, and the PRO + WAS group all exhibited bladder overactivity, presented as a shortened micturition interval and decreased threshold pressure evoking bladder contraction. The symptoms of the PRO group and the PRO + WAS group were more severe than those of the WAS group. The tissue staining results indicated that WAS itself caused only mild prostatic inflammation but could significantly aggravate chemical-induced prostatic inflammation, as well as the total number of mast cells and proportion of activated mast cells.
CONCLUSIONS: Our refined murine PRO model could manifest persistent bladder overactivity, pelvic hyperalgesia and prostatic inflammation. WAS could induce mild prostatic inflammation and aggravate primary prostatic inflammation.
摘要：
目标：迄今为止，很少有研究考虑心理因素对慢性前列腺炎（PRO）模型的影响。这里,我们的目的是改进结合化学诱导前列腺炎和心理压力的鼠PRO模型。
方法:将40只小鼠随机分为4组:正常对照组,PRO组,避水应激(WAS)组，和PRO+WAS组。将10只小鼠分配到每组:5只用于细胞图(CMG),5只用于vonFrey测试和组织学分析。PRO是通过前列腺注射10％多聚甲醛诱导的。将WAS小鼠置于中间平台上,每天1小时,连续10天。
结果：vonFrey试验的结果表明，WAS和PRO均可诱导小鼠膀胱痛觉过敏，WAS+PRO组也有明显的盆腔疼痛症状。CMG结果表明，PRO小组，WAS组,PRO+WAS组均表现为膀胱过度活动,表现为排尿间隔缩短和阈值压力降低引起膀胱收缩。PRO组和PRO+WAS组的症状较WAS组严重。组织染色结果表明，WAS本身仅引起轻度前列腺炎症，但可明显加重化学诱导的前列腺炎症。以及肥大细胞的总数和活化肥大细胞的比例。
结论：我们的精制鼠PRO模型可以表现出持续的膀胱过度活动，盆腔痛觉过敏和前列腺炎症。WAS可诱发轻度前列腺炎症，加重原发性前列腺炎症。
方法:将40只小鼠随机分为4组:正常对照组,PRO组,避水应激(WAS)组，和PRO+WAS组。将10只小鼠分配到每组:5只用于细胞图(CMG),5只用于vonFrey测试和组织学分析。PRO是通过前列腺注射10％多聚甲醛诱导的。将WAS小鼠置于中间平台上,每天1小时,连续10天。
结果：vonFrey试验的结果表明，WAS和PRO均可诱导小鼠膀胱痛觉过敏，WAS+PRO组也有明显的盆腔疼痛症状。CMG结果表明，PRO小组，WAS组,PRO+WAS组均表现为膀胱过度活动,表现为排尿间隔缩短和阈值压力降低引起膀胱收缩。PRO组和PRO+WAS组的症状较WAS组严重。组织染色结果表明，WAS本身仅引起轻度前列腺炎症，但可明显加重化学诱导的前列腺炎症。以及肥大细胞的总数和活化肥大细胞的比例。
结论：我们的精制鼠PRO模型可以表现出持续的膀胱过度活动，盆腔痛觉过敏和前列腺炎症。WAS可诱发轻度前列腺炎症，加重原发性前列腺炎症。
