Abstract：
BACKGROUND: Hypertension is associated with endothelial dysfunction. An imbalance in the production of Nitric Oxide (NO) and Reactive Oxygen Species (ROS), leading to impaired NO-cyclic Guanosine Monophosphate (cGMP) pathway, contributes to this disorder. Red Yeast Rice (RYR), produced from the fermentation of rice with Monascus purpureus, is a traditional functional food originating from China. Although recognized for its anti-dyslipidemia properties, there has been growing evidence regarding the anti-hypertensive effects of RYR. However, these studies only focused on its direct and short-term effects.
OBJECTIVE: This study aims to investigate the vasoprotective effects of chronic oral RYR administration using Spontaneously Hypertensive Rats (SHR).
METHODS: SHR were randomly divided into 3 groups: SHR - Control; SHR - RYR extract (100 mg/kg/day); SHR - lovastatin (10 mg/kg/day). Wistar-Kyoto Rats (WKY) were used as normotensive controls. All animals were treated for 12 weeks by oral gavage. Systolic Blood Pressure (SBP) was measured weekly (tail-cuff method). Vascular reactivity was determined using isolated rat aortic rings in an organ bath. Aortic ROS, NO, tetrahydrobiopterin (BH4 ), and cGMP levels were evaluated.
RESULTS: Administration of RYR attenuated SBP elevation and enhanced endothelium-dependent vasodilation in aortic rings. In addition, RYR decreased ROS production and significantly improved the level of vascular NO, BH4, and cGMP.
CONCLUSIONS: In an SHR model, treatment with RYR for 12 weeks exerts an SBP lowering effect that can be attributed to improved vascular function via reduction of oxidative stress, decreased endothelial NO Synthase (eNOS) uncoupling and enhanced NO-cGMP pathway.
OBJECTIVE: This study aims to investigate the vasoprotective effects of chronic oral RYR administration using Spontaneously Hypertensive Rats (SHR).
METHODS: SHR were randomly divided into 3 groups: SHR - Control; SHR - RYR extract (100 mg/kg/day); SHR - lovastatin (10 mg/kg/day). Wistar-Kyoto Rats (WKY) were used as normotensive controls. All animals were treated for 12 weeks by oral gavage. Systolic Blood Pressure (SBP) was measured weekly (tail-cuff method). Vascular reactivity was determined using isolated rat aortic rings in an organ bath. Aortic ROS, NO, tetrahydrobiopterin (BH4 ), and cGMP levels were evaluated.
RESULTS: Administration of RYR attenuated SBP elevation and enhanced endothelium-dependent vasodilation in aortic rings. In addition, RYR decreased ROS production and significantly improved the level of vascular NO, BH4, and cGMP.
CONCLUSIONS: In an SHR model, treatment with RYR for 12 weeks exerts an SBP lowering effect that can be attributed to improved vascular function via reduction of oxidative stress, decreased endothelial NO Synthase (eNOS) uncoupling and enhanced NO-cGMP pathway.
摘要：
背景：高血压与内皮功能障碍有关。一氧化氮（NO）和活性氧（ROS）产生的不平衡，导致NO-环磷酸鸟苷（cGMP）途径受损，有助于这种疾病。红酵母米（RYR），用紫红曲霉发酵大米，是原产于中国的传统功能性食品。尽管其抗血脂异常特性得到认可，越来越多的证据表明RYR的抗高血压作用.然而,这些研究只关注其直接和短期影响。
目的：本研究旨在研究自发性高血压大鼠（SHR）长期口服RYR的血管保护作用。
方法：SHR随机分为3组：SHR-对照组；SHR-RYR提取物（100mg/kg/天）；SHR-洛伐他汀（10mg/kg/天）。Wistar-Kyoto大鼠（WKY）用作正常血压对照。通过口服管饲法治疗所有动物12周。每周测量收缩压(SBP)(尾套法)。在器官浴中使用分离的大鼠主动脉环确定血管反应性。主动脉ROS,NO,四氢生物蝶呤(BH4),和cGMP水平进行评估。
结果：施用RYR减轻了SBP升高并增强了主动脉环中内皮依赖性血管舒张功能。此外,RYR降低了ROS的产生，显著提高了血管NO的水平，BH4和cGMP。
结论：在SHR模型中，用RYR治疗12周发挥SBP降低作用，可归因于通过减少氧化应激改善血管功能，降低内皮NO合成酶（eNOS）解偶联和增强NO-cGMP途径。
目的：本研究旨在研究自发性高血压大鼠（SHR）长期口服RYR的血管保护作用。
方法：SHR随机分为3组：SHR-对照组；SHR-RYR提取物（100mg/kg/天）；SHR-洛伐他汀（10mg/kg/天）。Wistar-Kyoto大鼠（WKY）用作正常血压对照。通过口服管饲法治疗所有动物12周。每周测量收缩压(SBP)(尾套法)。在器官浴中使用分离的大鼠主动脉环确定血管反应性。主动脉ROS,NO,四氢生物蝶呤(BH4),和cGMP水平进行评估。
结果：施用RYR减轻了SBP升高并增强了主动脉环中内皮依赖性血管舒张功能。此外,RYR降低了ROS的产生，显著提高了血管NO的水平，BH4和cGMP。
结论：在SHR模型中，用RYR治疗12周发挥SBP降低作用，可归因于通过减少氧化应激改善血管功能，降低内皮NO合成酶（eNOS）解偶联和增强NO-cGMP途径。
