Abstract：
OBJECTIVE: The incorporation of retromolar bone grafts used for alveolar ridge augmentation is not well understood. This prospective observational study aims to supply histomorphometrical data from bone graft biopsies taken at the time of retrieval and after a 3-month healing period using patient-matched biopsies.
METHODS: In 17 patients, trephine biopsies of the graft were acquired at the time of graft retrieval and after a 3-month healing period. The biopsies were compared histomorphometrically regarding the number of osteocytes, appearance of osteocyte lacunae, quantity, surface area, and activity of the Haversian canals.
RESULTS: All grafts appeared clinically stable after screw removal and 17 implants were placed. Histomorphometric analysis revealed no significant difference in the number of osteocytes (p = .413), osteocyte lacunae (p = .611), the ratio of filled/empty osteocyte lacunae (p = .467) and active Haversian canals (p = .495) between the biopsies retrieved after a 3-months healing period with those at the time of grafting. The only significant difference was noted in the mean surface area of the Haversian canals (p = .002). Specifically, the grafts post 3-month healing showed a significantly larger mean area (0.069 mm2) compared to the time of grafting (0.029 mm2).
CONCLUSIONS: This study demonstrates, compared to other data, a high rate of vital structures in retromolar bone block grafts after 3 months of healing, exhibiting the same histological features in comparison to the biopsies from the native alveolar ridge. Standard histomorphometrical parameters, e.g., the amount of filled or empty osteocyte lacunae for the description of the vitality of the graft need to be reappraised.
METHODS: In 17 patients, trephine biopsies of the graft were acquired at the time of graft retrieval and after a 3-month healing period. The biopsies were compared histomorphometrically regarding the number of osteocytes, appearance of osteocyte lacunae, quantity, surface area, and activity of the Haversian canals.
RESULTS: All grafts appeared clinically stable after screw removal and 17 implants were placed. Histomorphometric analysis revealed no significant difference in the number of osteocytes (p = .413), osteocyte lacunae (p = .611), the ratio of filled/empty osteocyte lacunae (p = .467) and active Haversian canals (p = .495) between the biopsies retrieved after a 3-months healing period with those at the time of grafting. The only significant difference was noted in the mean surface area of the Haversian canals (p = .002). Specifically, the grafts post 3-month healing showed a significantly larger mean area (0.069 mm2) compared to the time of grafting (0.029 mm2).
CONCLUSIONS: This study demonstrates, compared to other data, a high rate of vital structures in retromolar bone block grafts after 3 months of healing, exhibiting the same histological features in comparison to the biopsies from the native alveolar ridge. Standard histomorphometrical parameters, e.g., the amount of filled or empty osteocyte lacunae for the description of the vitality of the graft need to be reappraised.
摘要：
目的：磨牙后骨移植用于牙槽隆起的情况尚不清楚。这项前瞻性观察性研究旨在提供在检索时和3个月愈合期后使用患者匹配的活检进行的骨移植活检的组织形态计量学数据。
方法：在17例患者中，在移植物取出时和3个月的愈合期后获得移植物的环钻活检。在组织形态上比较活检的骨细胞数量，骨细胞腔隙的出现，数量,表面积,和哈弗斯运河的活动。
结果：移除螺钉后，所有移植物均表现出临床稳定，并放置了17个植入物。组织形态计量学分析显示骨细胞数量无显著差异（p=0.413），骨细胞腔隙（p=.611），3个月愈合期后获得的活检与移植时的活检之间的填充/空骨细胞腔隙（p=.467）和活跃的Haversian管（p=.495）的比率。唯一的显著差异是Haversian运河的平均表面积(p=.002)。具体来说,与移植时间（0.029mm2）相比，3个月愈合后的移植物显示出明显更大的平均面积（0.069mm2）。
结论：这项研究表明，与其他数据相比，在3个月的愈合后，磨牙后骨块移植物中重要结构的发生率很高，与天然牙槽骨的活检相比，表现出相同的组织学特征。标准组织形态计量学参数,例如，需要重新评估用于描述移植物活力的填充或空骨细胞空洞的数量。
方法：在17例患者中，在移植物取出时和3个月的愈合期后获得移植物的环钻活检。在组织形态上比较活检的骨细胞数量，骨细胞腔隙的出现，数量,表面积,和哈弗斯运河的活动。
结果：移除螺钉后，所有移植物均表现出临床稳定，并放置了17个植入物。组织形态计量学分析显示骨细胞数量无显著差异（p=0.413），骨细胞腔隙（p=.611），3个月愈合期后获得的活检与移植时的活检之间的填充/空骨细胞腔隙（p=.467）和活跃的Haversian管（p=.495）的比率。唯一的显著差异是Haversian运河的平均表面积(p=.002)。具体来说,与移植时间（0.029mm2）相比，3个月愈合后的移植物显示出明显更大的平均面积（0.069mm2）。
结论：这项研究表明，与其他数据相比，在3个月的愈合后，磨牙后骨块移植物中重要结构的发生率很高，与天然牙槽骨的活检相比，表现出相同的组织学特征。标准组织形态计量学参数,例如，需要重新评估用于描述移植物活力的填充或空骨细胞空洞的数量。
