Abstract：
Citrinin is a hepato-nephrotoxic mycotoxin produced by fungal species. The Monascus purpureus fungus plays a crucial role in the fermentation of red rice to produce red yeast rice-based food supplements, which represent the primary source of human exposure to citrinin. In this study, a simple and sensitive analytical method was successfully developed and validated for the citrinin determination in these products. The extraction process involved a QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) step and citrinin determination by ultra high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS). The proposed method provided satisfactory linearity, percentage of recovery from 82 to 104% with relative standard deviations (RSD) lower than 14%, and limits of detection and quantification of 0.07 μg/Kg and 0.24 μg/kg, respectively. Among the 14 samples analyzed, citrinin was found in two red rice samples (0.24 and 0.46 μg/kg) and in six food supplements (from 0.44 to 87 μg/kg).
摘要：
柑橘素是由真菌物种产生的肝肾毒性霉菌毒素。红曲霉真菌在红米发酵生产红曲米食品补充剂中起着至关重要的作用，这代表了人类接触citrinin的主要来源。在这项研究中,成功开发了一种简单而灵敏的分析方法，并对这些产品中的桔霉素进行了验证。提取过程涉及QuEChERS(Quick，Easy,便宜,有效,Rugged,和安全）步骤，并通过超高效液相色谱-串联质谱（UHPLC-MS/MS）进行citrinin测定。所提出的方法提供了令人满意的线性，回收率从82％到104％，相对标准偏差（RSD）低于14％，检测限和定量限为0.07μg/Kg和0.24μg/kg，分别。在分析的14个样本中，在两个红米样品（0.24和0.46μg/kg）和六个食品补充剂（从0.44至87μg/kg）中发现了citrinin。
