PDF(Pubmed)
Abstract：
Coxiella burnetii is the worldwide zoonotic infectious agent for Q fever in humans and animals. Farm animals are the main reservoirs of C. burnetii infection, which is mainly transmitted via tick bites. In humans, oral, percutaneous, and respiratory routes are the primary sources of infection transmission. The clinical signs vary from flu-like symptoms to endocarditis for humans\' acute and chronic Q fever. While it is usually asymptomatic in livestock, abortion, stillbirth, infertility, mastitis, and endometritis are its clinical consequences. Infected farm animals shed C. burnetii in birth products, milk, feces, vaginal mucus, and urine. Milk is an important source of infection among foods of animal origin. This study aimed to determine the prevalence and molecular characterization of C. burnetii in milk samples of dairy animals from two districts in Punjab, Pakistan, as it has not been reported there so far. Using a convenience sampling approach, the current study included 304 individual milk samples from different herds of cattle, buffalo, goats, and sheep present on 39 farms in 11 villages in the districts of Kasur and Lahore. PCR targeting the IS1111 gene sequence was used for its detection. Coxiella burnetii DNA was present in 19 of the 304 (6.3%) samples. The distribution was 7.2% and 5.2% in districts Kasur and Lahore, respectively. The results showed the distribution in ruminants as 3.4% in buffalo, 5.6% in cattle, 6.7% in goats, and 10.6% in sheep. From the univariable analysis, the clinical signs of infection i.e. mastitis and abortion were analyzed for the prevalence of Coxiella burnetii. The obtained sequences were identical to the previously reported sequence of a local strain in district Lahore, Sahiwal and Attock. These findings demonstrated that the prevalence of C. burnetii in raw milk samples deserves more attention from the health care system and veterinary organizations in Kasur and Lahore of Punjab, Pakistan. Future studies should include different districts and human populations, especially professionals working with animals, to estimate the prevalence of C. burnetii.
摘要：
伯氏柯西氏菌是人类和动物Q热的全球人畜共患病传染因子。农场动物是C.burnetii感染的主要宿主，主要通过蜱叮咬传播。在人类中,口服,经皮,和呼吸道是感染传播的主要来源。人类急性和慢性Q热的临床症状从流感样症状到心内膜炎不等。虽然它通常在牲畜中无症状，流产,死产,不孕症,乳腺炎,和子宫内膜炎是其临床后果。受感染的农场动物在分娩产品中脱落了C.burnetii，牛奶，粪便,阴道粘液,还有尿液.牛奶是动物源性食物中的重要感染源。本研究旨在确定旁遮普省两个地区的乳牛中的C.burnetii的患病率和分子特征。巴基斯坦,因为到目前为止还没有报道。使用一种方便的抽样方法，目前的研究包括来自不同牛群的304份个体牛奶样本，水牛,山羊,和羊出现在卡苏尔和拉合尔地区11个村庄的39个农场。将靶向IS1111基因序列的PCR用于其检测。304个样品中有19个（6.3％）存在伯氏柯西氏菌DNA。在卡苏尔和拉合尔地区的分布分别为7.2％和5.2％，分别。结果表明，反刍动物在水牛中的分布为3.4%，牛的5.6%，6.7%的山羊，绵羊占10.6%。从单变量分析来看，分析了感染的临床体征，即乳腺炎和流产的流行情况。获得的序列与先前报道的拉合尔地区当地菌株的序列相同，Sahiwal和Attock.这些发现表明，原奶样本中的C.burnetii的患病率值得旁遮普邦卡苏尔和拉合尔的医疗保健系统和兽医组织的更多关注，巴基斯坦。未来的研究应该包括不同的地区和人群，尤其是从事动物工作的专业人士，来估计C.burnetii的患病率。
