Abstract：
Due to the wide application of reporter gene-related visible/NIR-I bioluminescent imaging, multiplexed fluorescence imaging across visible/NIR-I/NIR-II has excellent potential in biomedical research. However, in vivo multiplexed imaging applications across those regions have rarely been reported due to the lack of proper fluorophores. Herein, nine squaraine dyes, which exhibit diverse adsorption and emission wavelengths, were synthesized. Among them, water-soluble SQ 710-5k and SQ 905 were found to have significant absorption differences, which allowed the tumor and lymph nodes to be identified. Then, for the first time, six-channel multiplexed fluorescence imaging across visible/NIR-I/II was achieved by coordination with reporter gene-related bioluminescent phosphors. Additional research revealed that SQ 710-5k exhibited higher-quality blood vessels and tumor imaging in NIR-II. H-aggregates SQ 905 demonstrated a high photothermal conversion efficiency for photothermal therapy. This study proposed an approach to creating small molecular dyes that coordinate with reporter gene-related bioluminescent phosphors for six-color fluorescence imaging.
摘要：
由于报告基因相关可见/NIR-I生物发光成像的广泛应用,跨可见光/NIR-I/NIR-II的多路荧光成像在生物医学研究中具有出色的潜力。然而,由于缺乏适当的荧光团，在这些区域的体内多路成像应用很少有报道。在这里，九种方酸染料，表现出不同的吸附和发射波长，是合成的。其中,发现水溶性SQ710-5k和SQ905具有显着的吸收差异，这使得肿瘤和淋巴结得以识别。然后，第一次,通过与报告基因相关的生物发光荧光粉协调，实现了可见光/NIR-I/II的六通道多重荧光成像。其他研究表明，SQ710-5k在NIR-II中表现出更高质量的血管和肿瘤成像。H-聚集体SQ905表现出用于光热治疗的高光热转换效率。这项研究提出了一种方法来创建与报告基因相关的生物发光磷光体协调的小分子染料，用于六色荧光成像。
