PDF(Pubmed)
Abstract：
UNASSIGNED: To test the hypothesis that (C-C motif) ligand 2 (CCL2) and CCL3 impact retinal function decline and inflammation during Staphylococcus aureus endophthalmitis.
UNASSIGNED: Experimental endophthalmitis was initiated by intravitreal injection of 5000 colony-forming units of S. aureus into the eyes of C57BL/6J, CCL2-/-, or CCL3-/- mice. At 12 and 24 hours post-infection, retinal function, bacterial load, and myeloperoxidase levels were quantified.
UNASSIGNED: During S. aureus endophthalmitis, we observed a significant improvement in retinal function in CCL2-/- mice relative to C57BL/6J mice at 12 hours but not at 24 hours. In CCL3-/- mice, retinal function was significantly improved relative to C57BL/6J mice at 12 and 24 hours. The absence of CCL2 did not alter intraocular S. aureus intraocular concentrations. However, CCL3-/- mice had significantly lower intraocular S. aureus at 12 hours but not at 24 hours. No difference in myeloperoxidase levels was observed between C57BL/6J and CCL2-/- mice at 12 hours. CCL3-/- mice had almost no myeloperoxidase at 12 hours. At 24 hours, increased myeloperoxidase was observed in CCL2-/- and CCL3-/- mice relative to C57BL/6J mice.
UNASSIGNED: Although the absence of CCL2 resulted in improved retinal function retention at 12 hours, CCL3 deficiency resulted in improved retinal function at 12 and 24 hours. CCL3 deficiency, but not CCL2 deficiency, resulted in almost no inflammation at 12 hours. However, at 24 hours, the absence of CCL2 or CCL3 resulted in significantly increased inflammation. These results suggest that, although both CCL2 and CCL3 impact intraocular infection outcomes, CCL3 may have a more significant impact in S. aureus endophthalmitis.
UNASSIGNED: Experimental endophthalmitis was initiated by intravitreal injection of 5000 colony-forming units of S. aureus into the eyes of C57BL/6J, CCL2-/-, or CCL3-/- mice. At 12 and 24 hours post-infection, retinal function, bacterial load, and myeloperoxidase levels were quantified.
UNASSIGNED: During S. aureus endophthalmitis, we observed a significant improvement in retinal function in CCL2-/- mice relative to C57BL/6J mice at 12 hours but not at 24 hours. In CCL3-/- mice, retinal function was significantly improved relative to C57BL/6J mice at 12 and 24 hours. The absence of CCL2 did not alter intraocular S. aureus intraocular concentrations. However, CCL3-/- mice had significantly lower intraocular S. aureus at 12 hours but not at 24 hours. No difference in myeloperoxidase levels was observed between C57BL/6J and CCL2-/- mice at 12 hours. CCL3-/- mice had almost no myeloperoxidase at 12 hours. At 24 hours, increased myeloperoxidase was observed in CCL2-/- and CCL3-/- mice relative to C57BL/6J mice.
UNASSIGNED: Although the absence of CCL2 resulted in improved retinal function retention at 12 hours, CCL3 deficiency resulted in improved retinal function at 12 and 24 hours. CCL3 deficiency, but not CCL2 deficiency, resulted in almost no inflammation at 12 hours. However, at 24 hours, the absence of CCL2 or CCL3 resulted in significantly increased inflammation. These results suggest that, although both CCL2 and CCL3 impact intraocular infection outcomes, CCL3 may have a more significant impact in S. aureus endophthalmitis.
摘要：
■检验（C-C基序）配体2（CCL2）和CCL3在金黄色葡萄球菌眼内炎期间影响视网膜功能下降和炎症的假设。
■实验性眼内炎通过玻璃体内注射5000个菌落形成单位的金黄色葡萄球菌进入C57BL/6J的眼睛，CCL2-/-,或CCL3-/-小鼠。感染后12和24小时，视网膜功能,细菌负荷，和髓过氧化物酶水平进行定量。
■在金黄色葡萄球菌眼内炎期间，我们观察到CCL2-/-小鼠的视网膜功能相对于C57BL/6J小鼠在12小时而不是在24小时显著改善。在CCL3-/-小鼠中，视网膜功能相对于C57BL/6J小鼠在12和24小时显著改善。缺乏CCL2不会改变眼内金黄色葡萄球菌的眼内浓度。然而,CCL3-/-小鼠在12小时具有显著较低的眼内金黄色葡萄球菌,而不是在24小时。在12小时时,在C57BL/6J和CCL2-/-小鼠之间没有观察到髓过氧化物酶水平的差异。CCL3-/-小鼠在12小时时几乎没有髓过氧化物酶。24小时后,相对于C57BL/6J小鼠,在CCL2-/-和CCL3-/-小鼠中观察到髓过氧化物酶增加。
■尽管缺乏CCL2可改善12小时时的视网膜功能保留，CCL3缺乏在12和24小时导致改善的视网膜功能。CCL3缺陷，但不是CCL2缺乏，在12小时时几乎没有炎症。然而,24小时后,CCL2或CCL3缺失导致炎症显著增加.这些结果表明,尽管CCL2和CCL3均影响眼内感染结局，CCL3可能在金黄色葡萄球菌眼内炎中具有更显著的影响。
■实验性眼内炎通过玻璃体内注射5000个菌落形成单位的金黄色葡萄球菌进入C57BL/6J的眼睛，CCL2-/-,或CCL3-/-小鼠。感染后12和24小时，视网膜功能,细菌负荷，和髓过氧化物酶水平进行定量。
■在金黄色葡萄球菌眼内炎期间，我们观察到CCL2-/-小鼠的视网膜功能相对于C57BL/6J小鼠在12小时而不是在24小时显著改善。在CCL3-/-小鼠中，视网膜功能相对于C57BL/6J小鼠在12和24小时显著改善。缺乏CCL2不会改变眼内金黄色葡萄球菌的眼内浓度。然而,CCL3-/-小鼠在12小时具有显著较低的眼内金黄色葡萄球菌,而不是在24小时。在12小时时,在C57BL/6J和CCL2-/-小鼠之间没有观察到髓过氧化物酶水平的差异。CCL3-/-小鼠在12小时时几乎没有髓过氧化物酶。24小时后,相对于C57BL/6J小鼠,在CCL2-/-和CCL3-/-小鼠中观察到髓过氧化物酶增加。
■尽管缺乏CCL2可改善12小时时的视网膜功能保留，CCL3缺乏在12和24小时导致改善的视网膜功能。CCL3缺陷，但不是CCL2缺乏，在12小时时几乎没有炎症。然而,24小时后,CCL2或CCL3缺失导致炎症显著增加.这些结果表明,尽管CCL2和CCL3均影响眼内感染结局，CCL3可能在金黄色葡萄球菌眼内炎中具有更显著的影响。
