Abstract：
In a previous study characterizing Campylobacter strains deficient in selenium metabolism, 50 strains were found to be similar to, but distinct from, the selenonegative species Campylobacter lanienae. Initial characterization based on multilocus sequence typing and the phylogeny of a set of 20 core genes determined that these strains form three putative taxa within the selenonegative cluster. A polyphasic study was undertaken here to further clarify their taxonomic position within the genus. The 50 selenonegative strains underwent phylogenetic analyses based on the sequences of the 16S rRNA gene and an expanded set of 330 core genes. Standard phenotypic testing was also performed. All strains were microaerobic and anaerobic, Gram-negative, spiral or curved cells with some displaying coccoid morphologies. Strains were motile, oxidase, catalase, and alkaline phosphatase positive, urease negative, and reduced nitrate. Strains within each clade had unique phenotypic profiles that distinguished them from other members of the genus. Core genome phylogeny clearly placed the 50 strains into three clades. Pairwise average nucleotide identity and digital DNA-DNA hybridization values were all below the recommended cut-offs for species delineation with respect to C. lanienae and other related Campylobacter species. The data presented here clearly show that these strains represent three novel species within the genus, for which the names Campylobacter devanensis sp. nov. (type strain RM3662T=LMG 33097T=NCTC 15074T), Campylobacter porcelli sp. nov. (type strain RM6137T=LMG 33098T=CCUG 77054T=NCTC 15075T) and Campylobacter vicugnae sp. nov. (type strain RM12175T=LMG 33099T=CCUG 77055T=NCTC 15076T) are proposed.
摘要：
在先前的研究中，描述了缺乏硒代谢的弯曲杆菌菌株，发现50个菌株类似，但不同于，硒阴性物种拉尼亚弯曲杆菌。基于多位点序列分型和一组20个核心基因的系统发育的初始表征确定，这些菌株在硒负簇内形成三个推定的分类群。在此进行了多相研究，以进一步阐明它们在属中的分类位置。根据16SrRNA基因的序列和扩展的330个核心基因，对50个硒阴性菌株进行了系统发育分析。还进行了标准表型测试。所有菌株均为微氧和厌氧，革兰氏阴性,螺旋或弯曲的细胞，有些细胞具有球形形态。菌株是活跃的，氧化酶,过氧化氢酶,碱性磷酸酶阳性，脲酶阴性，和还原的硝酸盐。每个进化枝中的菌株具有独特的表型特征，可将其与该属的其他成员区分开。核心基因组系统发育清楚地将50个菌株分为三个分支。关于C.lanienae和其他相关弯曲杆菌物种，成对平均核苷酸同一性和数字DNA-DNA杂交值均低于推荐的物种划分界限。这里提供的数据清楚地表明，这些菌株代表了该属中的三个新物种，为此命名为弯曲杆菌。11月。（类型菌株RM3662T=LMG33097T=NCTC15074T），弯曲杆菌。11月。（类型菌株RM6137T=LMG33098T=CCUG77054T=NCTC15075T）和维多利亚弯曲杆菌。11月。(型应变RM12175T=LMG33099T=CCUG77055T=NCTC15076T)提出。
