PDF(Pubmed)
Abstract：
Functionalized graphene oxide nanoparticles (NPs) have emerged as promising nanocarriers for drug delivery in lung cancer therapy. Quercetin and lurbinectedin encapsulated in graphene oxide (GO) NPs are tested for treating A549 lung cancer cells. Spectroscopic analyses show that graphene oxide functionalization creates a transparent, smooth surface for drug loading. Treatment with quercetin/lurbinectedin-loaded GO NPs induces notable cytotoxic effects in lung cancer cells, as evidenced by distinct morphological alterations and confirmed apoptotic cellular death observed through fluorescence microscopy. Additionally, our study highlights the impact of this approach on lung cancer metastasis, supported by qRT-PCR analysis of relative gene expression levels, including p53, Bax, Caspase-3, and Bcl 2, revealing robust molecular mechanisms underlying therapeutic efficacy against A549 and PC9 cell lines. Flow cytometric analyses further confirm the induction of cellular death in lung cancer cells following administration of the nanoformulation. Our findings show that quercetin/lurbinectedin-loaded GO NPs may be a promising lung cancer treatment, opening new avenues for targeted and effective therapies.
摘要：
功能化的氧化石墨烯纳米颗粒（NPs）已成为肺癌治疗中药物递送的有希望的纳米载体。测试封装在氧化石墨烯(GO)NP中的槲皮素和lurbinectedin用于治疗A549肺癌细胞。光谱分析表明，氧化石墨烯功能化创造了一个透明的，表面光滑的药物装载。用槲皮素/lurbintedin负载的GONP治疗在肺癌细胞中诱导显著的细胞毒性作用，通过荧光显微镜观察到明显的形态学改变和证实的凋亡细胞死亡证明。此外,我们的研究强调了这种方法对肺癌转移的影响，由相对基因表达水平的qRT-PCR分析支持，包括p53,Bax,Caspase-3和Bcl2，揭示了针对A549和PC9细胞系的治疗功效的强大分子机制。流式细胞术分析进一步证实在施用纳米制剂后诱导肺癌细胞中的细胞死亡。我们的研究结果表明，槲皮素/lurbinectedin负载的GONP可能是一种有前途的肺癌治疗，为有针对性的有效疗法开辟新的途径。
