PDF(Pubmed)
Abstract：
Cancer is the second most fatal disease among women. In recent years, utilizing strategies based on carbon quantum dots (CQDs) as targeted drug delivery systems has had a significant impact on advancing and improving cancer treatment. This study is focused on the development of a nanocarrier, based on CQDs, for improving the therapeutic efficiency of mitoxantrone (MTX). Hence, the N-doped CQDs were synthesized by a hydrothermal method. Following its purification, MTX was loaded to the CQD, resulting in an increase in the size from 36.78 ± 0.9 nm to 157.8 ± 12.18 nm, with an ideal drug entrapment efficiency of 97 %. Drug release investigation showed a pH-dependent improvement, from 8 % at pH 7.4 to 11 % at pH 5.2 after 48 h. Based on the Methylthiazolyldiphenyl-tetrazolium bromide (MTT) results after 5 h of treatment on MCF-7 breast cancer cells, the N-doped CQD showed no significant effect on the cancer cells, whereas a half maximal Inhibitory Concentration (IC50) was achieved with the N-doped CQD-MTX complex at a concentration between 0.5 to 0.8 μM. Therefore, the newly developed drug delivery complex was capable of providing a rather identical influence on MCF-7 cells, as the free MTX, however, improving the pharmacokinetic of the drug by its controlled and on-target drug release, due to an alteration in distribution and absorption parameters.
摘要：
癌症是女性第二大致命疾病。近年来,利用基于碳量子点(CQDs)的策略作为靶向药物递送系统对推进和改善癌症治疗产生了重大影响.这项研究的重点是纳米载体的开发，基于CQDs，用于提高米托蒽醌（MTX）的治疗效率。因此，通过水热法合成了N掺杂的CQDs。净化后，MTX被加载到CQD,导致尺寸从36.78±0.9nm增加到157.8±12.18nm，理想的药物包封率为97%。药物释放调查显示pH依赖性改善，在48小时后，从pH7.4的8%到pH5.2的11%。基于对MCF-7乳腺癌细胞处理5小时后的甲基噻唑基二苯基-四唑溴化物（MTT）结果，N掺杂CQD对癌细胞无显著影响,而N掺杂的CQD-MTX复合物在0.5至0.8μM的浓度下达到一半最大抑制浓度(IC50)。因此,新开发的药物递送复合物能够对MCF-7细胞提供相当相同的影响，作为免费的MTX，然而,通过控制和靶向药物释放来改善药物的药代动力学，由于分布和吸收参数的变化。
