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Abstract：
Introduction: Pogostemon cablin (PC) is used in traditional Chinese medicine and food, as it exerts pharmacological effects, such as immune-modulatory, antibacterial, antioxidant, antitumor, and antiviral. Currently, the pharmacokinetics (PK) studies of PC mainly focus on individual components. However, research on these individual components cannot reflect the actual PK characteristics of PC after administration. Therefore, the simultaneous determination of multiple components in rat plasma using UPLC-MS/MS was used for the pharmacokinetic study after oral administration of PC extract in this study, providing reference value for the clinical application of PC. Methods: In the present study, a reliable and sensitive ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous determination of 15 prototype components (vanillic acid, vitexin, verbascoside, isoacteoside, hyperoside, cosmosiin, apigenin, β-rhamnocitrin, acacetin, ombuin, pogostone, pachypodol, vicenin-2, retusin, and diosmetin-7-O-β-D-glucopyranoside) in rat plasma after oral administration of the PC extract. Plasma samples were prepared via protein precipitation using acetonitrile, and icariin was used as the internal standard (IS). Results: The intra-day and inter-day accuracies ranged from -12.0 to 14.3%, and the precision of the analytes was less than 11.3%. The extraction recovery rate of the analytes ranged from 70.6-104.5%, and the matrix effects ranged from 67.4-104.8%. Stability studies proved that the analytes were stable under the tested conditions, with a relative standard deviation lower than 14.1%. Conclusion: The developed method can be applied to evaluate the PK of 15 prototype components in PC extracts of rats after oral administration using UPLC-MS/MS, providing valuable information for the development and clinical safe, effective, and rational use of PC.
摘要：
简介：Pogostemoncablin（PC）用于中药和食品，因为它发挥药理作用，比如免疫调节，抗菌，抗氧化剂，抗肿瘤，和抗病毒。目前,PC的药代动力学（PK）研究主要集中在单个成分上。然而,对这些单独成分的研究不能反映PC给药后的实际PK特性。因此,本研究采用UPLC-MS/MS同时测定大鼠血浆中多种成分,为PC的临床应用提供参考价值。方法：在本研究中，建立了一种可靠而灵敏的超高效液相色谱/串联质谱（UPLC-MS/MS）方法，并对其同时测定15种原型成分（香草酸，vitexin,Verbascoside,异麦角苷,金丝桃苷,宇宙素，芹菜素,β-鼠李糖苷，acacetin,ombuin,pogestone,pachypodol,vicenin-2retusin,口服PC提取物后，大鼠血浆中的薯蛋白-7-O-β-D-吡喃葡萄糖苷）。血浆样品通过使用乙腈的蛋白质沉淀来制备,并使用淫羊藿苷作为内标(IS)。结果：日内和日间准确度范围为-12.0至14.3%，分析物的精密度小于11.3%。分析物的提取回收率为70.6-104.5%，基质效应在67.4-104.8%之间。稳定性研究证明，分析物在测试条件下是稳定的，相对标准偏差低于14.1%。结论:所建立的方法可用于UPLC-MS/MS评估大鼠口服PC提取物中15种原型成分的PK。为开发和临床安全提供有价值的信息，有效,合理使用PC。
