PDF(Pubmed)
Abstract：
Microsatellites, known as simple sequence repeats (SSRs), are short tandem repeats of 1 to 6 nucleotide motifs found in all genomes, particularly eukaryotes. They are widely used as co-dominant markers in genetic analyses and molecular breeding. Triticeae, a tribe of grasses, includes major cereal crops such as bread wheat, barley, and rye, as well as abundant forage and lawn grasses, playing a crucial role in global food production and agriculture. To enhance genetic work and expedite the improvement of Triticeae crops, we have developed TriticeaeSSRdb, an integrated and user-friendly database. It contains 3,891,705 SSRs from 21 species and offers browsing options based on genomic regions, chromosomes, motif types, and repeat motif sequences. Advanced search functions allow personalized searches based on chromosome location and length of SSR. Users can also explore the genes associated with SSRs, design customized primer pairs for PCR validation, and utilize practical tools for whole-genome browsing, sequence alignment, and in silico SSR prediction from local sequences. We continually update TriticeaeSSRdb with additional species and practical utilities. We anticipate that this database will greatly facilitate trait genetic analyses and enhance molecular breeding strategies for Triticeae crops. Researchers can freely access the database at http://triticeaessrdb.com/.
摘要：
微卫星,称为简单序列重复(SSR)，是在所有基因组中发现的1到6个核苷酸基序的短串联重复，尤其是真核生物。它们被广泛用作遗传分析和分子育种中的共显性标记。小麦,一个部落的草，包括主要的谷类作物，如面包小麦，大麦,还有黑麦,以及丰富的牧草和草坪草，在全球粮食生产和农业中发挥着至关重要的作用。为了加强遗传工作，加快小麦作物的改良，我们开发了TriticeaeSSRdb，一个集成和用户友好的数据库。它包含来自21个物种的3,891,705个SSR，并提供基于基因组区域的浏览选项，染色体,主题类型，和重复基序序列。高级搜索功能允许基于SSR的染色体位置和长度的个性化搜索。用户还可以探索与SSR相关的基因，设计用于PCR验证的定制引物对，并利用实用工具进行全基因组浏览，序列比对,以及从局部序列进行计算机SSR预测。我们不断更新TriticeaeSSRdb与其他物种和实用工具。我们预计该数据库将极大地促进小麦作物的性状遗传分析并增强分子育种策略。研究人员可以自由访问数据库http://triticeaessrdb.com/。
