PDF(Pubmed)
Abstract：
This study aimed to demonstrate the existence of a possible correlation between the level of liver fluke infection in cattle caused by Fasciola hepatica and the titers of anti-parasite antibodies in the blood. 113 blood samples were taken from non-dewormed pasture cattle in the communal slaughterhouse of the Jijel city in order to determine the serological titration of anti-fluke antibodies by the ELISA method. After slaughtering the animals studied, a count of parasites present in the inspected livers was carried out in order to assess the level of parasitic infection. The prevalence of bovine fasciolosis (due to Fasciola hepatica) based on counting the number of flukes in the inspected livers was around 61.06%, while the seroprevalence obtained is 55.75%. The sensitivity and specificity of the ELISA test used in this study were, respectively, 91.3% and 100%. A very strong correlation (r = 0.93 to p < 0.0001) was statistically demonstrated between the antibody titers and the number of flukes in the inspected livers. According to the results of this study, there is a strong statistical correlation between serum antibody titers and the number of flukes present in the liver (the higher the number of parasites in the liver, the higher the level of anti-flukes antibodies in the blood).
摘要：
这项研究旨在证明肝片吸虫引起的牛肝吸虫感染水平与血液中抗寄生虫抗体滴度之间可能存在相关性。从Jijel市公共屠宰场的非驱虫放牧牛抽取113份血液样本，以通过ELISA方法确定抗吸虫抗体的血清学滴定。在屠杀了被研究的动物后，为了评估寄生虫感染的水平，对被检查肝脏中存在的寄生虫进行了计数。根据检查的肝脏中的吸虫数量计算，牛筋膜病（由于肝片吸虫）的患病率约为61.06%，而获得的血清阳性率为55.75%。本研究采用的ELISA检测的灵敏度和特异度,分别,91.3%和100%。在所检查的肝脏中,在抗体滴度和吸虫数量之间在统计学上证明了非常强的相关性(r=0.93至p<0.0001)。根据这项研究的结果,血清抗体滴度与肝脏中存在的吸虫数量之间存在很强的统计相关性（肝脏中寄生虫的数量越高，血液中抗吸虫抗体的水平越高）。
