PDF(Pubmed)
Abstract：
UNASSIGNED: The effect of local corticosteroid (CS) injections on rotator cuff muscles remains poorly defined, despite the significance of muscle quality as a crucial prognostic factor for patients with rotator cuff tears (RCTs).
UNASSIGNED: To compare alterations in gene and protein expression patterns in the rotator cuff muscles of patients with RCTs who received frequent joint CS injections with alterations in those without a history of CS injections.
UNASSIGNED: Controlled laboratory study.
UNASSIGNED: A total of 24 rotator cuff muscle samples with medium-sized tears from 12 patients with a frequent joint CS injection history (steroid group; 7 men and 5 women who had received ≥5 injections with at least 1 within the previous 3 months; mean age, 63.0 ± 7.2 years) and 12 age- and sex-matched control patients without a history of CS injections (no-steroid group) were acquired. Alterations in the expression of genes and proteins associated with adipogenesis, myogenesis, inflammation, and muscle fibrosis were compared between the groups using quantitative reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemistry. Statistical analysis included comparison of group means using the Mann-Whitney U test, chi-square test, or Fisher exact test and logistic regression for multivariate analysis.
UNASSIGNED: In the steroid group, the mRNA expression levels of adipogenic CCAAT/enhancer-binding protein alpha (C/EBPα; P = .008) and muscle atrophy-related genes (atrogin; P = .019) were significantly higher, and those of myogenic differentiation 1 (MyoD; P = .035), inflammatory interleukin 6 (IL-6; P = .035), and high mobility group box 1 (P = .003) were significantly lower compared with the no-steroid group. In addition, MyoD (P = .041) and IL-6 (P = .026) expression were decreased in the steroid versus no-steroid group. Immunohistochemistry revealed increased expression of C/EBPα and atrogin and decreased expression of MyoD and IL-6 in the steroid versus no-steroid group.
UNASSIGNED: Patients with RCTs and a history of frequent CS injections exhibited an upregulation of adipogenic and muscle atrophy-related genes and proteins within the rotator cuff muscles and a downregulation in the expression of myogenic and inflammatory genes and proteins in the same muscles.
UNASSIGNED: These altered gene and protein expressions by frequent local CS injections may cause poor outcomes in patients with RCTs.
UNASSIGNED: To compare alterations in gene and protein expression patterns in the rotator cuff muscles of patients with RCTs who received frequent joint CS injections with alterations in those without a history of CS injections.
UNASSIGNED: Controlled laboratory study.
UNASSIGNED: A total of 24 rotator cuff muscle samples with medium-sized tears from 12 patients with a frequent joint CS injection history (steroid group; 7 men and 5 women who had received ≥5 injections with at least 1 within the previous 3 months; mean age, 63.0 ± 7.2 years) and 12 age- and sex-matched control patients without a history of CS injections (no-steroid group) were acquired. Alterations in the expression of genes and proteins associated with adipogenesis, myogenesis, inflammation, and muscle fibrosis were compared between the groups using quantitative reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemistry. Statistical analysis included comparison of group means using the Mann-Whitney U test, chi-square test, or Fisher exact test and logistic regression for multivariate analysis.
UNASSIGNED: In the steroid group, the mRNA expression levels of adipogenic CCAAT/enhancer-binding protein alpha (C/EBPα; P = .008) and muscle atrophy-related genes (atrogin; P = .019) were significantly higher, and those of myogenic differentiation 1 (MyoD; P = .035), inflammatory interleukin 6 (IL-6; P = .035), and high mobility group box 1 (P = .003) were significantly lower compared with the no-steroid group. In addition, MyoD (P = .041) and IL-6 (P = .026) expression were decreased in the steroid versus no-steroid group. Immunohistochemistry revealed increased expression of C/EBPα and atrogin and decreased expression of MyoD and IL-6 in the steroid versus no-steroid group.
UNASSIGNED: Patients with RCTs and a history of frequent CS injections exhibited an upregulation of adipogenic and muscle atrophy-related genes and proteins within the rotator cuff muscles and a downregulation in the expression of myogenic and inflammatory genes and proteins in the same muscles.
UNASSIGNED: These altered gene and protein expressions by frequent local CS injections may cause poor outcomes in patients with RCTs.
摘要：
■局部皮质类固醇（CS）注射对肩袖肌肉的影响尚不明确，尽管肌肉质量作为肩袖撕裂（RCT）患者的关键预后因素具有重要意义。
■比较接受频繁联合CS注射的RCT患者肩袖肌肉基因和蛋白质表达模式的改变与没有CS注射史的患者的改变。
■对照实验室研究。
■总共24个肩袖肌肉样本，来自12名频繁关节CS注射史的患者（类固醇组；7名男性和5名女性，在过去3个月内至少接受过5次注射；平均年龄，获得63.0±7.2岁）和12名年龄和性别匹配的对照组患者，没有CS注射史（无类固醇组）。与脂肪生成相关的基因和蛋白质表达的变化，肌生成，炎症,使用定量逆转录-聚合酶链反应比较各组之间的肌肉纤维化，西方印迹,和免疫组织化学。统计分析包括使用Mann-WhitneyU检验比较组均值，卡方检验,或Fisher精确检验和多变量分析的逻辑回归。
■在类固醇组中，成脂CCAAT/增强子结合蛋白α(C/EBPα;P=.008)和肌肉萎缩相关基因(atrogin;P=.019)的mRNA表达水平显著增高,和肌源性分化1（MyoD；P=.035），炎性白细胞介素6（IL-6；P=.035），与无类固醇组相比，高迁移率组1（P=0.003）显着降低。此外,与无类固醇组相比，类固醇组的MyoD（P=.041）和IL-6（P=.026）表达降低。免疫组织化学显示，类固醇与非类固醇组的C/EBPα和atrogin表达增加，MyoD和IL-6表达降低。
■患有RCT并有频繁CS注射史的患者表现出肩袖肌肉内成脂肪和肌肉萎缩相关基因和蛋白质的上调，以及肌源性和炎症基因的表达下调。相同的肌肉和蛋白质。
■通过频繁的局部CS注射这些改变的基因和蛋白质表达可能导致RCT患者的不良预后。
■比较接受频繁联合CS注射的RCT患者肩袖肌肉基因和蛋白质表达模式的改变与没有CS注射史的患者的改变。
■对照实验室研究。
■总共24个肩袖肌肉样本，来自12名频繁关节CS注射史的患者（类固醇组；7名男性和5名女性，在过去3个月内至少接受过5次注射；平均年龄，获得63.0±7.2岁）和12名年龄和性别匹配的对照组患者，没有CS注射史（无类固醇组）。与脂肪生成相关的基因和蛋白质表达的变化，肌生成，炎症,使用定量逆转录-聚合酶链反应比较各组之间的肌肉纤维化，西方印迹,和免疫组织化学。统计分析包括使用Mann-WhitneyU检验比较组均值，卡方检验,或Fisher精确检验和多变量分析的逻辑回归。
■在类固醇组中，成脂CCAAT/增强子结合蛋白α(C/EBPα;P=.008)和肌肉萎缩相关基因(atrogin;P=.019)的mRNA表达水平显著增高,和肌源性分化1（MyoD；P=.035），炎性白细胞介素6（IL-6；P=.035），与无类固醇组相比，高迁移率组1（P=0.003）显着降低。此外,与无类固醇组相比，类固醇组的MyoD（P=.041）和IL-6（P=.026）表达降低。免疫组织化学显示，类固醇与非类固醇组的C/EBPα和atrogin表达增加，MyoD和IL-6表达降低。
■患有RCT并有频繁CS注射史的患者表现出肩袖肌肉内成脂肪和肌肉萎缩相关基因和蛋白质的上调，以及肌源性和炎症基因的表达下调。相同的肌肉和蛋白质。
■通过频繁的局部CS注射这些改变的基因和蛋白质表达可能导致RCT患者的不良预后。
