Abstract：
Sclareol, a diterpene alcohol, is the most common starting material for the synthesis of ambrox, which serves as a sustainable substitute for ambergris, a valuable fragrance secreted by sperm whales. Sclareol has also been proposed to possess antibacterial, antifungal, and anticancer activities. However, in nature, sclareol is only produced by a few plant species, including Cistus creticus, Cleome spinosa, Nicotiana glutinosa, and Salvia sclarea, which limits its commercial application. In this study, we cloned the two genes responsible for sclareol biosynthesis in S. sclarea, labda-13-en-8-ol diphosphate synthase (LPPS) and sclareol synthase (SS), and overexpressed them in tobacco (Nicotiana tabacum L.). The best transgenic tobacco lines accumulated 4.1 μg/cm2 of sclareol, which is comparable to the sclareol production of N. glutinosa, a natural sclareol producer. Thus, sclareol synthesis in tobacco represents a potential alternative means for the production of this high-value compound.
摘要：
香雷醇,一种二萜醇，是合成Ambrox最常见的原料，作为龙涎香的可持续替代品，抹香鲸分泌的有价值的香味。还提出了香雷醇具有抗菌性，抗真菌药,和抗癌活动。然而,在自然界中,紫苏醇只由几种植物产生，包括Cistuscreticus,Cleomespinosa,烟草，和丹参巩膜，限制了其商业应用。在这项研究中,我们在S.sclarea中克隆了负责sclareol生物合成的两个基因，labda-13-en-8-醇二磷酸合酶（LPPS）和香紫苏醇合酶（SS），并在烟草中过度表达(烟草)。最好的转基因烟草系积累了4.1μg/cm2的香紫苏醇，这与丁香酵母的香雷醇产量相当，一个天然的紫苏醇生产商.因此，烟草中的香紫苏醇合成代表了生产这种高价值化合物的潜在替代手段。
