Abstract：
Steroids stand for a class of hormones (natural and synthetic) known to be helpful for a number of disorders. Despite the aforementioned beneficial effects of using these hormones, anabolic-androgenic steroids (AAS) are also widely abused in a non-therapeutic manner for muscle-building and strength-increasing properties that may lead to genotoxicity in different tissues. The present study aims to understand whether genotoxicity may be a suitable biomarker for AAS exposure in vivo in both experimental animal and human studies. All studies published in PubMed/Medline, Scopus, and Web of Science electronic databases that presented data on DNA damage caused by AAS were analyzed. A total of 15 articles were included in this study, and after thoroughly reviewing the studies, a total of 8 articles were classified as Strong, 6 were classified as Moderate, and only 1 was classified as Weak, totaling 14 studies being considered either Strong or Moderate. This classification makes it possible to consider the present findings as reliable. The meta-analysis data revealed a statistically significant difference in Wistar rat testis cells with AAS compared to control for tail length and % tail DNA (p < 0.001), so that the selected articles were considered homogeneous and the I2 of 0% indicated low heterogeneity. In summary, genotoxicity can be considered a suitable biomarker for monitoring AAS exposure as a result of DNA breakage and oxidative DNA damage.
摘要：
类固醇代表一类已知有助于许多疾病的激素（天然和合成）。尽管使用这些激素有上述有益效果，合成代谢-雄激素类固醇（AAS）也以非治疗方式被广泛滥用，用于增强肌肉和增加力量的特性，这可能导致不同组织的遗传毒性。本研究旨在了解遗传毒性是否可能是实验动物和人类研究中体内AAS暴露的合适生物标志物。发表在PubMed/Medline上的所有研究，Scopus,和WebofScience电子数据库提供了有关AAS引起的DNA损伤的数据进行了分析。本研究共纳入15篇文章，在彻底回顾研究后，共有8篇文章被归类为“坚强”，6人被归类为中度，只有1个被归类为“弱”,共有14项研究被认为是强的或中等的。这种分类使得可以认为目前的发现是可靠的。荟萃分析数据显示，与对照组相比，使用AAS的Wistar大鼠睾丸细胞在尾部长度和尾部DNA百分比方面存在统计学上的显着差异（p<0.001），因此，选定的文章被认为是同质的，I2为0%表示低异质性。总之,遗传毒性可以被认为是监测由于DNA断裂和氧化性DNA损伤而导致的AAS暴露的合适生物标志物。
