Abstract：
OBJECTIVE: To evaluate the effect of Ziziphus honey on the healing of post-extraction alveolar sockets by estimating the levels of osteopontin (OPN) in humans.
METHODS: Randomised controlled trial. Place and Duration of the Study: Dental section of the Lahore General Hospital, Lahore, Pakistan, from March 2020 to February 2021.
METHODS: A total of 30 patients were included in the study. The mean age was 35 ± 0.28 years. The participants were adults undergoing permanent molar extraction, randomly divided into two groups, a control group and an experimental group. After tooth extractions in both groups, 1ml of Ziziphus honey was administered into the extracted tooth socket of the experimental group while no intervention was done to the control group. Saliva samples were collected on day 0 before tooth extraction and on days 3 and 7 after tooth extractions. Enzyme-linked immunosorbent assay (ELISA) technique was used to measure the levels of OPN in the saliva sample. Radiographic evaluation was also done with the help of periapical radiographs using Image J® software. To find out the significance of the outcome in experimental and control groups, an unpaired t-test was applied. A p-value <0.05 was considered statistically significant.
RESULTS: A total of 30 participants were selected for the study, of which 16 were females and 14 were males. The OPN levels between the control vs. experimental groups were (22.55 ± 2.45 vs. 23.31 ± 2.38; p = 0.4) on day 0, (30.95 ± 2.96 vs. 53.29 ± 4.69; p = 0.001) on day 3, and (55.33 ± 4.52 vs. 81.90 ± 4.49; p = 0.001) on day 7.
CONCLUSIONS: Increased salivary levels of the OPN in the experimental group with the use of Ziziphus honey suggests better bone healing as compared to the control group.
BACKGROUND: Extraction tooth, Honey, Osteopontin, Ziziphus, Bone healing.
METHODS: Randomised controlled trial. Place and Duration of the Study: Dental section of the Lahore General Hospital, Lahore, Pakistan, from March 2020 to February 2021.
METHODS: A total of 30 patients were included in the study. The mean age was 35 ± 0.28 years. The participants were adults undergoing permanent molar extraction, randomly divided into two groups, a control group and an experimental group. After tooth extractions in both groups, 1ml of Ziziphus honey was administered into the extracted tooth socket of the experimental group while no intervention was done to the control group. Saliva samples were collected on day 0 before tooth extraction and on days 3 and 7 after tooth extractions. Enzyme-linked immunosorbent assay (ELISA) technique was used to measure the levels of OPN in the saliva sample. Radiographic evaluation was also done with the help of periapical radiographs using Image J® software. To find out the significance of the outcome in experimental and control groups, an unpaired t-test was applied. A p-value <0.05 was considered statistically significant.
RESULTS: A total of 30 participants were selected for the study, of which 16 were females and 14 were males. The OPN levels between the control vs. experimental groups were (22.55 ± 2.45 vs. 23.31 ± 2.38; p = 0.4) on day 0, (30.95 ± 2.96 vs. 53.29 ± 4.69; p = 0.001) on day 3, and (55.33 ± 4.52 vs. 81.90 ± 4.49; p = 0.001) on day 7.
CONCLUSIONS: Increased salivary levels of the OPN in the experimental group with the use of Ziziphus honey suggests better bone healing as compared to the control group.
BACKGROUND: Extraction tooth, Honey, Osteopontin, Ziziphus, Bone healing.
摘要：
目的：通过估计人体中骨桥蛋白（OPN）的水平来评估Ziziphus蜂蜜对拔牙后肺泡窝愈合的影响。
方法：随机对照试验。研究的地点和持续时间：拉合尔总医院牙科科，拉合尔,巴基斯坦,从2020年3月到2021年2月。
方法：本研究共纳入30例患者。平均年龄为35±0.28岁。参与者是接受永久性磨牙拔除的成年人，随机分为两组,对照组和实验组。两组拔牙后，实验组拔牙牙槽内给予1ml酸枣仁蜂蜜，对照组不干预。在拔牙前第0天和拔牙后第3天和第7天收集唾液样品。采用酶联免疫吸附测定(ELISA)技术测定唾液样品中OPN的含量。还在使用ImageJ®软件的根尖周射线照片的帮助下进行射线照相评估。为了找出实验组和对照组的结果的意义，采用非配对t检验。P值<0.05被认为是统计学上显著的。
结果：总共选择了30名参与者进行研究，其中女性16人，男性14人。控制与之间的OPN水平实验组为(22.55±2.45vs.23.31±2.38；p=0.4）在第0天，（30.95±2.96vs.第3天的53.29±4.69；p=0.001）和（55.33±4.52vs.第7天81.90±4.49；p=0.001）。
结论：与对照组相比，使用Ziziphus蜂蜜的实验组中OPN的唾液水平增加表明骨愈合更好。
背景：拔牙，亲爱的,骨桥蛋白,齐齐弗,骨愈合。
方法：随机对照试验。研究的地点和持续时间：拉合尔总医院牙科科，拉合尔,巴基斯坦,从2020年3月到2021年2月。
方法：本研究共纳入30例患者。平均年龄为35±0.28岁。参与者是接受永久性磨牙拔除的成年人，随机分为两组,对照组和实验组。两组拔牙后，实验组拔牙牙槽内给予1ml酸枣仁蜂蜜，对照组不干预。在拔牙前第0天和拔牙后第3天和第7天收集唾液样品。采用酶联免疫吸附测定(ELISA)技术测定唾液样品中OPN的含量。还在使用ImageJ®软件的根尖周射线照片的帮助下进行射线照相评估。为了找出实验组和对照组的结果的意义，采用非配对t检验。P值<0.05被认为是统计学上显著的。
结果：总共选择了30名参与者进行研究，其中女性16人，男性14人。控制与之间的OPN水平实验组为(22.55±2.45vs.23.31±2.38；p=0.4）在第0天，（30.95±2.96vs.第3天的53.29±4.69；p=0.001）和（55.33±4.52vs.第7天81.90±4.49；p=0.001）。
结论：与对照组相比，使用Ziziphus蜂蜜的实验组中OPN的唾液水平增加表明骨愈合更好。
背景：拔牙，亲爱的,骨桥蛋白,齐齐弗,骨愈合。
