PDF(Pubmed)
Abstract：
BACKGROUND: The auxin/indole-3-acetic acid (Aux/IAA) gene family is a crucial element of the auxin signaling pathway, significantly influencing plant growth and development. Hence, we conducted a comprehensive investigation of Aux/IAAs gene family using the Sp75 and Monoe-Viroflay genomes in spinach.
RESULTS: A total of 24 definitive Aux/IAA genes were identified, exhibiting diverse attributes in terms of amino acid length, molecular weight, and isoelectric points. This diversity underscores potential specific roles within the family, such as growth regulation and stress response. Structural analysis revealed significant variations in gene length and molecular weight. These variations indicate distinct roles within the Aux/IAA gene family. Chromosomal distribution analysis exhibited a dispersed pattern, with chromosomes 4 and 1 hosting the highest and lowest numbers of Aux/IAA genes, respectively. Phylogenetic analysis grouped the identified genes into distinct clades, revealing potential evolutionary relationships. Notably, the phylogenetic tree highlighted specific gene clusters suggesting shared genetic ancestry and potential functional synergies within spinach. Expression analysis under NAA treatment unveiled gene-specific and time-dependent responses, with certain genes exhibiting distinct temporal expression patterns. Specifically, SpoIAA5 displayed a substantial increase at 2 h post-NAA treatment, while SpoIAA7 and SpoIAA9 demonstrated continuous rises, peaking at the 4-hour time point.
CONCLUSIONS: These observations indicate a complex interplay of gene-specific and temporal regulation in response to auxin. Moreover, the comparison with other plant species emphasized both shared characteristics and unique features in Aux/IAA gene numbers, providing insights into the evolutionary dynamics of this gene family. This comprehensive characterization of Aux/IAA genes in spinach not only establishes the foundation for understanding their specific functions in spinach development but also provides a valuable resource for experimental validation and further exploration of their roles in the intricate network of auxin signaling pathways.
RESULTS: A total of 24 definitive Aux/IAA genes were identified, exhibiting diverse attributes in terms of amino acid length, molecular weight, and isoelectric points. This diversity underscores potential specific roles within the family, such as growth regulation and stress response. Structural analysis revealed significant variations in gene length and molecular weight. These variations indicate distinct roles within the Aux/IAA gene family. Chromosomal distribution analysis exhibited a dispersed pattern, with chromosomes 4 and 1 hosting the highest and lowest numbers of Aux/IAA genes, respectively. Phylogenetic analysis grouped the identified genes into distinct clades, revealing potential evolutionary relationships. Notably, the phylogenetic tree highlighted specific gene clusters suggesting shared genetic ancestry and potential functional synergies within spinach. Expression analysis under NAA treatment unveiled gene-specific and time-dependent responses, with certain genes exhibiting distinct temporal expression patterns. Specifically, SpoIAA5 displayed a substantial increase at 2 h post-NAA treatment, while SpoIAA7 and SpoIAA9 demonstrated continuous rises, peaking at the 4-hour time point.
CONCLUSIONS: These observations indicate a complex interplay of gene-specific and temporal regulation in response to auxin. Moreover, the comparison with other plant species emphasized both shared characteristics and unique features in Aux/IAA gene numbers, providing insights into the evolutionary dynamics of this gene family. This comprehensive characterization of Aux/IAA genes in spinach not only establishes the foundation for understanding their specific functions in spinach development but also provides a valuable resource for experimental validation and further exploration of their roles in the intricate network of auxin signaling pathways.
摘要：
背景：生长素/吲哚-3-乙酸（Aux/IAA）基因家族是生长素信号通路的关键元件，显著影响植物生长发育。因此，我们使用菠菜中的Sp75和Monoe-Viroflay基因组对Aux/IAAs基因家族进行了全面调查。
结果：共鉴定出24个确定的Aux/IAA基因，在氨基酸长度方面表现出不同的属性，分子量,和等电点。这种多样性强调了家庭中潜在的特定角色，如生长调节和应激反应。结构分析显示基因长度和分子量的显着变化。这些变异表明Aux/IAA基因家族中的不同作用。染色体分布分析显示出分散的模式，4号和1号染色体承载着最高和最低数量的Aux/IAA基因，分别。系统发育分析将鉴定的基因分为不同的进化枝，揭示潜在的进化关系。值得注意的是,系统发育树突出了特定的基因簇，表明菠菜中具有共同的遗传血统和潜在的功能协同作用。NAA处理下的表达分析揭示了基因特异性和时间依赖性反应，某些基因表现出不同的时间表达模式。具体来说,SpotifyIAA5在NAA治疗后2小时显示出大幅增加，而SpotifyIAA7和SpotifyIAA9表现出持续的上升，在4小时的时间点达到峰值。
结论：这些观察结果表明，在对生长素的反应中，基因特异性和时间调节之间存在复杂的相互作用。此外，与其他植物物种的比较强调了Aux/IAA基因数量的共同特征和独特特征，提供对这个基因家族的进化动态的见解。这种对菠菜中Aux/IAA基因的全面表征不仅为理解其在菠菜发育中的特定功能奠定了基础，而且为实验验证和进一步探索其在复杂的生长素信号通路网络中的作用提供了宝贵的资源。
结果：共鉴定出24个确定的Aux/IAA基因，在氨基酸长度方面表现出不同的属性，分子量,和等电点。这种多样性强调了家庭中潜在的特定角色，如生长调节和应激反应。结构分析显示基因长度和分子量的显着变化。这些变异表明Aux/IAA基因家族中的不同作用。染色体分布分析显示出分散的模式，4号和1号染色体承载着最高和最低数量的Aux/IAA基因，分别。系统发育分析将鉴定的基因分为不同的进化枝，揭示潜在的进化关系。值得注意的是,系统发育树突出了特定的基因簇，表明菠菜中具有共同的遗传血统和潜在的功能协同作用。NAA处理下的表达分析揭示了基因特异性和时间依赖性反应，某些基因表现出不同的时间表达模式。具体来说,SpotifyIAA5在NAA治疗后2小时显示出大幅增加，而SpotifyIAA7和SpotifyIAA9表现出持续的上升，在4小时的时间点达到峰值。
结论：这些观察结果表明，在对生长素的反应中，基因特异性和时间调节之间存在复杂的相互作用。此外，与其他植物物种的比较强调了Aux/IAA基因数量的共同特征和独特特征，提供对这个基因家族的进化动态的见解。这种对菠菜中Aux/IAA基因的全面表征不仅为理解其在菠菜发育中的特定功能奠定了基础，而且为实验验证和进一步探索其在复杂的生长素信号通路网络中的作用提供了宝贵的资源。
