Abstract：
OBJECTIVE: To investigate the effects of oral exposure to iron oxide nanoparticles(Fe_2O_3NPs) on the reproductive system of male rats.
METHODS: Forty male SD rats were randomly divided into control group and low, medium, high dose groups, 10 rats in each group, normal saline and 50, 100 and 200 mg/kg Fe_2O_3NPs suspension were given by gavage, respectively. The volume of gavage was 10 mL/kg for 28 days. The body weight was weighed every three days, and the body weight changes of rats were recorded. After intraperitoneal anesthesia with 10% chloral hydrate, the rats were sacrificed by cervical dislocation, and the testis and epididymis were collected. Weigh and calculate the testicular coefficient and epididymal coefficient, the pathological sections of rat testis were observed by hematoxylin-eosin staining, the number of epididymal sperm was counted under an optical microscope and the sperm deformity rate was calculated. The activities of acid phosphatase(ACP), alkaline phosphatase(AKP), lactate dehydrogenase(LDH) and γ-glutamyl transpeptidase(γ-GT), the activity of superoxide dismutase(SOD), and the contents of glutathione(GSH) and malondialdehyde(MDA) in rat testis homogenate were detected by kit method.
RESULTS: Compared with control group, there was no significant difference in body weight, testicular coefficient and epididymal coefficient in each dose group. In the medium and high dose groups, the arrangement of spermatogenic epithelium was disordered and spermatogenic cells decreased. The number of sperm in high dose group was decreased, and the sperm deformity rate in medium and high dose groups was increased(P<0.01). The activity of ACP in medium and high dose groups increased(P<0.05), and the activity of γ-GT decreased(P<0.01). There was no significant change in the activity of AKP and LDH in testicular homogenate of rats in each group(P>0.05). The level of GSH in medium dose group was increased(P<0.05), and the content of MDA in medium and high dose groups was increased(P<0.01). There was no significant difference in SOD activity among the groups(P>0.05).
CONCLUSIONS: Under the conditions of this experiment, Fe_2O_3NPs can cause damage to the structure of rat testicular tissue, reduce the number of sperm, increase the rate of sperm deformity, interfere with the activity of marker enzymes in testicular tissue and induce oxidative stress injury, which has a negative impact on the reproductive system of male rats.
METHODS: Forty male SD rats were randomly divided into control group and low, medium, high dose groups, 10 rats in each group, normal saline and 50, 100 and 200 mg/kg Fe_2O_3NPs suspension were given by gavage, respectively. The volume of gavage was 10 mL/kg for 28 days. The body weight was weighed every three days, and the body weight changes of rats were recorded. After intraperitoneal anesthesia with 10% chloral hydrate, the rats were sacrificed by cervical dislocation, and the testis and epididymis were collected. Weigh and calculate the testicular coefficient and epididymal coefficient, the pathological sections of rat testis were observed by hematoxylin-eosin staining, the number of epididymal sperm was counted under an optical microscope and the sperm deformity rate was calculated. The activities of acid phosphatase(ACP), alkaline phosphatase(AKP), lactate dehydrogenase(LDH) and γ-glutamyl transpeptidase(γ-GT), the activity of superoxide dismutase(SOD), and the contents of glutathione(GSH) and malondialdehyde(MDA) in rat testis homogenate were detected by kit method.
RESULTS: Compared with control group, there was no significant difference in body weight, testicular coefficient and epididymal coefficient in each dose group. In the medium and high dose groups, the arrangement of spermatogenic epithelium was disordered and spermatogenic cells decreased. The number of sperm in high dose group was decreased, and the sperm deformity rate in medium and high dose groups was increased(P<0.01). The activity of ACP in medium and high dose groups increased(P<0.05), and the activity of γ-GT decreased(P<0.01). There was no significant change in the activity of AKP and LDH in testicular homogenate of rats in each group(P>0.05). The level of GSH in medium dose group was increased(P<0.05), and the content of MDA in medium and high dose groups was increased(P<0.01). There was no significant difference in SOD activity among the groups(P>0.05).
CONCLUSIONS: Under the conditions of this experiment, Fe_2O_3NPs can cause damage to the structure of rat testicular tissue, reduce the number of sperm, increase the rate of sperm deformity, interfere with the activity of marker enzymes in testicular tissue and induce oxidative stress injury, which has a negative impact on the reproductive system of male rats.
摘要：
目的：观察纳米氧化铁(Fe_2O_3NPs)口服对雄性大鼠生殖系统的影响。
方法:雄性SD大鼠40只,中等,高剂量组，每组10只，灌胃给予生理盐水和50、100和200mg/kgFe_2O_3NPs悬液，分别。灌胃量为10mL/kg,持续28天。体重每三天称重一次,记录大鼠体重变化。用10%水合氯醛腹腔麻醉后，大鼠被颈椎脱位处死,收集了睾丸和附睾。称量并计算睾丸系数和附睾系数，苏木精-伊红染色观察大鼠睾丸病理切片，在光学显微镜下计数附睾精子数量并计算精子畸形率。酸性磷酸酶（ACP）的活性，碱性磷酸酶(AKP),乳酸脱氢酶（LDH）和γ-谷氨酰转肽酶（γ-GT），超氧化物歧化酶（SOD）的活性，用试剂盒法检测大鼠睾丸匀浆中谷胱甘肽(GSH)和丙二醛(MDA)的含量。
结果：与对照组相比，体重没有显着差异，各剂量组睾丸系数、附睾系数。在中、高剂量组中,生精上皮排列紊乱，生精细胞减少。高剂量组精子数量减少,中、高剂量组精子畸形率升高(P<0.01)。中、高剂量组ACP活性升高(P<0.05),γ-GT活性下降(P<0.01)。各组年夜鼠睾丸匀浆中AKP和LDH活性无显著变更(P>0.05)。中剂量组GSH水平升高(P<0.05),中、高剂量组MDA含量升高(P<0.01)。各组间SOD活性差异无统计学意义(P>0.05)。
结论：在本实验条件下，Fe_2O_3NPs可引起大鼠睾丸组织结构的损伤,减少精子的数量，增加精子畸形率，干扰睾丸组织中标记酶的活性，诱导氧化应激损伤，这对雄性大鼠的生殖系统有负面影响。
方法:雄性SD大鼠40只,中等,高剂量组，每组10只，灌胃给予生理盐水和50、100和200mg/kgFe_2O_3NPs悬液，分别。灌胃量为10mL/kg,持续28天。体重每三天称重一次,记录大鼠体重变化。用10%水合氯醛腹腔麻醉后，大鼠被颈椎脱位处死,收集了睾丸和附睾。称量并计算睾丸系数和附睾系数，苏木精-伊红染色观察大鼠睾丸病理切片，在光学显微镜下计数附睾精子数量并计算精子畸形率。酸性磷酸酶（ACP）的活性，碱性磷酸酶(AKP),乳酸脱氢酶（LDH）和γ-谷氨酰转肽酶（γ-GT），超氧化物歧化酶（SOD）的活性，用试剂盒法检测大鼠睾丸匀浆中谷胱甘肽(GSH)和丙二醛(MDA)的含量。
结果：与对照组相比，体重没有显着差异，各剂量组睾丸系数、附睾系数。在中、高剂量组中,生精上皮排列紊乱，生精细胞减少。高剂量组精子数量减少,中、高剂量组精子畸形率升高(P<0.01)。中、高剂量组ACP活性升高(P<0.05),γ-GT活性下降(P<0.01)。各组年夜鼠睾丸匀浆中AKP和LDH活性无显著变更(P>0.05)。中剂量组GSH水平升高(P<0.05),中、高剂量组MDA含量升高(P<0.01)。各组间SOD活性差异无统计学意义(P>0.05)。
结论：在本实验条件下，Fe_2O_3NPs可引起大鼠睾丸组织结构的损伤,减少精子的数量，增加精子畸形率，干扰睾丸组织中标记酶的活性，诱导氧化应激损伤，这对雄性大鼠的生殖系统有负面影响。
