PDF(Pubmed)
Abstract：
Immunoglobulin A (IgA) is notable for its broad specificity toward multiple bacteria. Phosphorylcholine (PC) plays a role in the infection of pathogenic bacteria carrying PC and in the induction of IgA responses in the host immune system. The commercially available mouse monoclonal IgA, TEPC15-IgA, is a distinctive antibody with specificity for PC, warranting further exploration of its response to PC-bearing enteric bacteria. In this study, using 17 different enteric bacteria, including 3 aerobic and 14 anerobic bacteria that could be cultured in vitro, we confirmed that TEPC15-IgA recognizes 4 bacterial species: Lactobacillus taiwanensis, Limosilactobacillus frumenti, Streptococcus infantis, and Escherichia coli, although reactivity varied. Interestingly, TEPC15-IgA did not react with four of six Lactobacillus species used. Moreover, distinct target molecules associated with PC in L. taiwanensis and L. frumenti were evident, differing in molecular weight. These findings suggest that the natural generation of PC-specific IgA could prevent PC-mediated infections and potentially facilitate the formation of a microflora rich in indigenous bacteria with PC, particularly in the gastrointestinal tract.
摘要：
免疫球蛋白A（IgA）以其对多种细菌的广泛特异性而著称。磷酸胆碱（PC）在携带PC的病原菌的感染和宿主免疫系统中IgA反应的诱导中起作用。市售小鼠单克隆IgA，TEPC15-IgA,是一种对PC具有特异性的独特抗体，有必要进一步探索其对携带PC的肠道细菌的反应。在这项研究中,使用17种不同的肠道细菌，包括3个需氧菌和14个可在体外培养的厌氧菌，我们证实TEPC15-IgA可识别4种细菌：台湾乳杆菌，frumentiLimosilactobacillus,婴儿链球菌,和大肠杆菌，尽管反应性各不相同。有趣的是,TEPC15-IgA不与使用的六种乳杆菌属中的四种反应。此外，不同的目标分子与PC在台湾乳杆菌和L.frumenti是明显的，分子量不同。这些发现表明，PC特异性IgA的自然产生可以防止PC介导的感染，并可能促进富含PC的本地细菌的微生物群的形成。尤其是在胃肠道。
