Abstract：
This study aims to investigate the antiallergic effects of Shiikuwasha (Citrus depressa Hayata) leaf and peel extracts by examining the regulation of degranulation and inflammatory cytokine production from rat basophilic leukemia (RBL-2H3) cells and antigen-specific antibody production in sensitized mouse spleen lymphocytes. In vivo antiallergic activity was evaluated using the passive cutaneous anaphylaxis (PCA) reaction model. Extracts of Shiikuwasha leaves and peel were prepared using 80% methanol and dissolved in dimethylsulfoxide. The dinitrophenyl-human serum albumin-induced β-hexosaminidase levels in immunoglobulin (Ig) E-sensitized RBL-2H3 cells were assessed using enzymatic assays. Cytokine production was measured by enzyme-linked immunosorbent assay. Antibody production capacity was evaluated using lymphocytes isolated from spleens of type I allergy model mice. Lymphocytes were cultured for 72 h with Shiikuwasha extracts, and ovalbumin-specific IgE, IgG1, and IgG2a levels were measured. Shiikuwasha leaf and peel extract significantly reduced β-hexosaminidase release and suppressed interleukin-4 and tumor necrosis factor-α production from RBL-2H3 cells. Ovalbumin-specific IgE and IgG1 production decreased in Shiikuwasha extract-treated lymphocytes. These extracts also significantly suppressed the PCA reaction. Shiikuwasha leaf and peel extract reduce degranulation in RBL-2H3 cells and antibody production in spleen-derived lymphocytes and therefore exhibit antiallergic effects.
摘要：
本研究旨在通过研究大鼠嗜碱性白血病（RBL-2H3）细胞的脱颗粒和炎性细胞因子产生的调节以及致敏小鼠脾淋巴细胞中抗原特异性抗体的产生，研究Shiikuwasha（CitrusdepressaHayata）叶和皮提取物的抗过敏作用。使用被动皮肤过敏反应（PCA）反应模型评估了体内抗过敏活性。使用80％甲醇制备Shiikuwasha叶和果皮的提取物，并溶解在二甲基亚砜中。使用酶测定法评估了免疫球蛋白（Ig）E致敏的RBL-2H3细胞中二硝基苯-人血清白蛋白诱导的β-己糖胺酶水平。通过酶联免疫吸附测定来测量细胞因子的产生。使用从I型过敏模型小鼠的脾脏分离的淋巴细胞评估抗体生产能力。淋巴细胞与Shiikuwasha提取物一起培养72小时，和卵白蛋白特异性IgE，测量IgG1和IgG2a水平。Shiikuwasha叶和果皮提取物显着减少了β-己糖胺酶的释放，并抑制了RBL-2H3细胞产生的白介素4和肿瘤坏死因子α。在Shikuwasha提取物处理的淋巴细胞中，卵清蛋白特异性IgE和IgG1的产生减少。这些提取物还显著抑制了PCA反应。Shiikuwasha叶和果皮提取物减少了RBL-2H3细胞中的脱粒和脾脏衍生淋巴细胞中的抗体产生，因此表现出抗过敏作用。
