Abstract：
OBJECTIVE: To determine whether combining cross-linked (CL) collagen-integrated xenogeneic bone blocks stabilized with the fixation of resorbable collagen membranes (CM) can enhance guided bone regeneration (GBR) in the overaugmented calvarial defect model.
METHODS: Four circular defects with a diameter of 8 mm were prepared in the calvarium of 13 rabbits. Defects were randomly assigned to receive one of the following treatments: (i) non-cross-linked (NCL) porcine-derived collagen-embedded bone block covered by a CM without fixation (NCL + unfix group); (ii) NCL bone block covered by CM with fixation using bone-tack (NCL + fix group); (iii) cross-linked (CL) porcine-derived collagen-embedded bone block covered by CM without fixation (CL + unfix group); and (iv) CL bone block covered by CM with fixation using bone-tack fixation (CL + fix group). The efficacy of GBR was assessed through histological and molecular analyses after 2 and 8 weeks.
RESULTS: At 2 weeks, there were no significant differences in histologically measured areas of newly formed bone among the groups. At 8 weeks, however, the CL + fix group exhibited a larger area of new bone (5.08 ± 1.09 mm2, mean ± standard deviation) compared to the NCL + unfix (1.62 ± 0.42 mm2; p < .0083), NCL + fix (3.97 ± 1.39 mm2) and CL + unfix (2.55 ± 1.04 mm2) groups. Additionally, the expression levels of tumour necrosis factor-alpha, fibroblast growth factor-2, vascular endothelial growth factor, osteocalcin and calcitonin receptor were significantly higher in the CL + fix group compared to the other three groups (p < .0083).
CONCLUSIONS: Cross-linked bone blocks stabilized with collagen membrane fixation can significantly enhance GBR.
METHODS: Four circular defects with a diameter of 8 mm were prepared in the calvarium of 13 rabbits. Defects were randomly assigned to receive one of the following treatments: (i) non-cross-linked (NCL) porcine-derived collagen-embedded bone block covered by a CM without fixation (NCL + unfix group); (ii) NCL bone block covered by CM with fixation using bone-tack (NCL + fix group); (iii) cross-linked (CL) porcine-derived collagen-embedded bone block covered by CM without fixation (CL + unfix group); and (iv) CL bone block covered by CM with fixation using bone-tack fixation (CL + fix group). The efficacy of GBR was assessed through histological and molecular analyses after 2 and 8 weeks.
RESULTS: At 2 weeks, there were no significant differences in histologically measured areas of newly formed bone among the groups. At 8 weeks, however, the CL + fix group exhibited a larger area of new bone (5.08 ± 1.09 mm2, mean ± standard deviation) compared to the NCL + unfix (1.62 ± 0.42 mm2; p < .0083), NCL + fix (3.97 ± 1.39 mm2) and CL + unfix (2.55 ± 1.04 mm2) groups. Additionally, the expression levels of tumour necrosis factor-alpha, fibroblast growth factor-2, vascular endothelial growth factor, osteocalcin and calcitonin receptor were significantly higher in the CL + fix group compared to the other three groups (p < .0083).
CONCLUSIONS: Cross-linked bone blocks stabilized with collagen membrane fixation can significantly enhance GBR.
摘要：
目的：确定是否将交联（CL）胶原整合的异种骨块与可吸收胶原膜（CM）的固定相结合可以增强过度扩张的颅骨缺损模型中的引导骨再生（GBR）。
方法：在13只兔子的颅骨中制备了四个直径为8mm的圆形缺损。缺损被随机分配接受以下治疗之一：（i）未固定的CM覆盖的非交联（NCL）猪源性胶原包埋骨块（NCLunfix组）；（ii）使用骨钉固定的CM覆盖的NCL骨块（NCL固定组）；（iii）交联的（CL）猪源性胶原包埋骨块不固定的CM（CL固定）；2周和8周后通过组织学和分子分析评估GBR的功效。
结果：在2周时，各组间新形成骨的组织学测量面积无显著差异.在8周的时候,然而,与NCL+unfix（1.62±0.42mm2；p<.0083）相比，CL+fix组显示出更大的新骨面积（5.08±1.09mm2，平均值±标准偏差），NCL+fix(3.97±1.39mm2)和CL+unfix(2.55±1.04mm2)组。此外,肿瘤坏死因子-α的表达水平，成纤维细胞生长因子-2,血管内皮生长因子,与其他三组相比，CL+fix组的骨钙蛋白和降钙素受体显著高于其他三组(p<.0083)。
结论：用胶原膜固定稳定的交联骨块可显著增强GBR。
方法：在13只兔子的颅骨中制备了四个直径为8mm的圆形缺损。缺损被随机分配接受以下治疗之一：（i）未固定的CM覆盖的非交联（NCL）猪源性胶原包埋骨块（NCLunfix组）；（ii）使用骨钉固定的CM覆盖的NCL骨块（NCL固定组）；（iii）交联的（CL）猪源性胶原包埋骨块不固定的CM（CL固定）；2周和8周后通过组织学和分子分析评估GBR的功效。
结果：在2周时，各组间新形成骨的组织学测量面积无显著差异.在8周的时候,然而,与NCL+unfix（1.62±0.42mm2；p<.0083）相比，CL+fix组显示出更大的新骨面积（5.08±1.09mm2，平均值±标准偏差），NCL+fix(3.97±1.39mm2)和CL+unfix(2.55±1.04mm2)组。此外,肿瘤坏死因子-α的表达水平，成纤维细胞生长因子-2,血管内皮生长因子,与其他三组相比，CL+fix组的骨钙蛋白和降钙素受体显著高于其他三组(p<.0083)。
结论：用胶原膜固定稳定的交联骨块可显著增强GBR。
