Abstract：
Diesel degradation and bacterial growth were investigated in soil, marine water, and freshwater ecosystems using Acinetobacter baumannii IITG19, Providencia vermicola IITG20, and their mixed culture. Both bacteria were found to be effective in all three ecosystems, with the best degradation occurring in freshwater. Acinetobacter baumannii IITG19 showed higher degradation (59%, 62%, and 76%) than Providencia vermicola IITG20 (31%, 57%, and 67%) in soil, marine water, and freshwater, respectively. Alkanes showed higher degradation than naphthenes and aromatics for both strains. The mixed culture showed higher diesel degradation efficiency than individual strains in all ecosystems. The overall degradation was similar in soil and marine water (66%), while freshwater showed the highest degradation of 81%. In the presence of the mixed culture, the degradation of alkanes was more than 90%. Bacterial growth was highest in freshwater and lowest in soil for both bacteria and the mixed culture. Metabolite analysis confirmed alcoholic degradation for alkanes and cyclo-alcoholic degradation for naphthenes. The degradation rate for mixed culture was higher than that of both the individual strains. The mixed culture had highest degradation rate constant in freshwater at 0.11 day-1 followed by that in marine ecosystem at 0.078 day-1. The rate constant was lowest for soil ecosystem at 0.066 day-1. Thus the mixed culture showed effectiveness in all three ecosystems, with its highest effectiveness observed in the freshwater ecosystem.
摘要：
在土壤中研究了柴油降解和细菌生长，海水,和使用鲍曼不动杆菌IITG19，ProvidenciavermicolaIITG20及其混合培养物的淡水生态系统。发现这两种细菌在所有三个生态系统中都有效，最好的降解发生在淡水中。鲍曼不动杆菌IITG19表现出更高的降解(59%，62%,和76%)比普罗维登西亚维米科拉IITG20(31%，57%,和67%）在土壤中，海水,淡水,分别。两种菌株的烷烃降解均高于环烷烃和芳烃。在所有生态系统中，混合培养物显示出比单个菌株更高的柴油降解效率。土壤和海水的总体降解相似(66%)，而淡水的降解率最高，达81%。在混合文化的存在下，烷烃的降解率在90%以上。对于细菌和混合培养物，淡水中的细菌生长最高，土壤中的细菌生长最低。代谢物分析证实了烷烃的醇降解和环烷烃的环醇降解。混合培养的降解率高于两个单独菌株的降解率。混合培养物在淡水中的降解速率常数为0.11天-1，其次是海洋生态系统中的降解速率常数为0.078天-1。土壤生态系统的速率常数在0.066天1时最低。因此，混合培养在所有三个生态系统中都显示出有效性，在淡水生态系统中观察到最高的有效性。
