PDF(Pubmed)
Abstract：
Blood transfusions save lives and improve health every day. Despite the matching of blood types being stricter than it ever has been, emergency transfusions among incompatible blood types are still inevitable in the clinic when there is a lack of acceptable blood types for recipients. Here to overcome this, a counter measure nanoplatform consisting of a polymeric core coated by a red blood cell (RBC) membrane is developed. With A-type or B-type RBC membrane camouflaging, the nanoplatform is capable of specifically capturing anti-A or anti-B IgM antibodies within B-type or A-type whole blood, thereby decreasing the corresponding IgM antibody levels and then allowing the incompatible blood transfusions. In addition to IgM, the anti-RBC IgG antibody in a passive immunization murine model can likewise be neutralized by this nanoplatform, leading to prolonged circulation time of incompatible donor RBCs. Noteworthily, nanoplatform made by expired RBCs (>42 days stored hypothermically) and then subjected to lyophilization does not impair their effect on antibody neutralization. Most importantly, antibody-captured RBC-NP do not exacerbate the risk of inflammation, complement activation, and coagulopathy in an acute hemorrhagic shock murine model. Overall, this biomimetic nanoplatform can safely neutralize the antibody to enable incompatible blood transfusion.
摘要：
输血每天都能挽救生命并改善健康。尽管血型匹配比以往任何时候都更严格，当接受者缺乏可接受的血型时，在临床上仍然不可避免地进行不相容血型的紧急输血。为了克服这一点，开发了一种由涂覆有红细胞（RBC）膜的聚合物核组成的计数器纳米平台。用A型或B型红细胞膜伪装，纳米平台能够在B型或A型全血中特异性捕获抗A或抗BIgM抗体，从而降低相应的IgM抗体水平，然后允许不相容的输血。除了IgM,被动免疫小鼠模型中的抗RBCIgG抗体同样可以被该纳米平台中和，导致不相容供体红细胞的循环时间延长。值得注意的是,由过期的RBC(低温储存>42天)制备并随后进行冻干的纳米平台不损害其对抗体中和的作用。最重要的是,抗体捕获的RBC-NP不会加剧炎症的风险，补体激活,急性失血性休克小鼠模型的凝血障碍。总的来说,这种仿生纳米平台可以安全地中和抗体，使不相容的输血。
