PDF(Pubmed)
Abstract：
BACKGROUND: Medical dressings are designed to promote wound healing and reduce infection. The aim of project is to investigate the effect of natural brown colored cotton dressings on the healing of infected wounds in E.coli animals.
METHODS: In this study, degreased white cotton gauze was used as the control group, with degreased brown cotton gauze and degreased bleached brown cotton gauze as the experimental group 1 and experimental group 2, to investigate the effect on the repair of post-infectious wound damage in animals by establishing an infected wound model in rats with E.coli as the infecting organism.
RESULTS: The ability to promote healing of infected wounds was investigated by analyzing the wound healing status, macroscopic wound healing rate, hematoxylin-eosin staining, Masson staining, secretion of inflammatory factors by Elisa assay. The result showed that at day 14 of wound healing, the macroscopic wound healing rate was greater than 98% for all three groups of dressings; the collagen content reached 49.85 ± 5.84% in the experimental group 1 and 53.48 ± 5.32% in the experimental group 2, which was higher than the control group; brown cotton gauze promotes skin wound healing by shortening the inflammatory period in both groups. The expression of three inflammatory factors THF-α, IL-2, and IL-8 and three cytokines MMP-3, MMP-8, and MMP-9 were lower than that of the control group.
CONCLUSIONS: It was found that natural brown cotton gauze has better repairing and promoting healing effect on infected wounds. It opens up the application of natural brown cotton gauze in the treatment of infected wounds.
METHODS: In this study, degreased white cotton gauze was used as the control group, with degreased brown cotton gauze and degreased bleached brown cotton gauze as the experimental group 1 and experimental group 2, to investigate the effect on the repair of post-infectious wound damage in animals by establishing an infected wound model in rats with E.coli as the infecting organism.
RESULTS: The ability to promote healing of infected wounds was investigated by analyzing the wound healing status, macroscopic wound healing rate, hematoxylin-eosin staining, Masson staining, secretion of inflammatory factors by Elisa assay. The result showed that at day 14 of wound healing, the macroscopic wound healing rate was greater than 98% for all three groups of dressings; the collagen content reached 49.85 ± 5.84% in the experimental group 1 and 53.48 ± 5.32% in the experimental group 2, which was higher than the control group; brown cotton gauze promotes skin wound healing by shortening the inflammatory period in both groups. The expression of three inflammatory factors THF-α, IL-2, and IL-8 and three cytokines MMP-3, MMP-8, and MMP-9 were lower than that of the control group.
CONCLUSIONS: It was found that natural brown cotton gauze has better repairing and promoting healing effect on infected wounds. It opens up the application of natural brown cotton gauze in the treatment of infected wounds.
摘要：
背景：医用敷料旨在促进伤口愈合并减少感染。本项目旨在研究天然棕色棉敷料对大肠杆菌感染创面愈合的影响。
方法：在本研究中，以脱脂白色棉纱布为对照组,以脱脂棕色棉纱布和脱脂漂白棕色棉纱布作为实验1组和实验2组,通过建立以大肠杆菌为感染生物的大鼠感染创面模型,探讨其对动物感染后创面损伤的修复效果。
结果：通过分析伤口愈合状况，研究了促进感染伤口愈合的能力，宏观伤口愈合率，苏木精-伊红染色，Masson染色,用Elisa法检测炎症因子的分泌情况。结果显示在伤口愈合的第14天,3组敷料的宏观创面愈合率均大于98%；实验组1的胶原含量达到49.85±5.84%，实验组2的胶原含量达到53.48±5.32%，高于对照组；棕色棉纱布通过缩短炎症期来促进皮肤创面愈合。三种炎症因子THF-α的表达,IL-2、IL-8和三种细胞因子MMP-3、MMP-8、MMP-9均低于对照组。
结论：天然棕色棉纱布对感染创面有较好的修复和促进愈合作用。开辟了天然棕色棉纱布在沾染创面医治中的运用。
方法：在本研究中，以脱脂白色棉纱布为对照组,以脱脂棕色棉纱布和脱脂漂白棕色棉纱布作为实验1组和实验2组,通过建立以大肠杆菌为感染生物的大鼠感染创面模型,探讨其对动物感染后创面损伤的修复效果。
结果：通过分析伤口愈合状况，研究了促进感染伤口愈合的能力，宏观伤口愈合率，苏木精-伊红染色，Masson染色,用Elisa法检测炎症因子的分泌情况。结果显示在伤口愈合的第14天,3组敷料的宏观创面愈合率均大于98%；实验组1的胶原含量达到49.85±5.84%，实验组2的胶原含量达到53.48±5.32%，高于对照组；棕色棉纱布通过缩短炎症期来促进皮肤创面愈合。三种炎症因子THF-α的表达,IL-2、IL-8和三种细胞因子MMP-3、MMP-8、MMP-9均低于对照组。
结论：天然棕色棉纱布对感染创面有较好的修复和促进愈合作用。开辟了天然棕色棉纱布在沾染创面医治中的运用。
