Abstract：
Lichen planopilaris (LPP) and frontal fibrosing alopecia (FFA) are primary cicatricial alopecia that cause a major impact on quality of life due to irreversible hair loss and symptoms as itching, burning and pain. They are characterized by permanent loss of hair follicle stem cells (HFSCs) by pathomechanisms still poorly understood, resulting in poor efficacy of currently available treatments. Caveolae are flask-shaped lipid rafts invaginated within the plasma membrane of multiple cell types. Although their role in the HF physiology and pathophysiology is relatively unknown, we have previously demonstrated that the primary structural component of caveolae (caveolin-1 or Cav1) is upregulated in FFA. Thus, we propose to investigate the expression and localization of caveolae-associated structural proteins (Cav1, Cav2, and Cavin-1) and HFSCs (identified by K15) in both LPP and FFA. We analyzed 4 patients with LPP biopsied in affected and non-affected (NA) scalp, 4 patients with FFA biopsied in affected scalp and 4 healthy controls. Affected scalp of LPP and FFA demonstrated increased levels of Cav1 and Cavin-1 compared with HC and LPP-NA. Moreover, Cav1, Cav2 and Cavin1 all exhibit high colocalization with K15 and their expression appears to be negatively correlated, supporting the hypothesis that these proteins are important players in LPP/FFA and may serve as therapeutic targets in future treatments.
摘要：
扁平苔藓（LPP）和额叶纤维化脱发（FFA）是原发性瘢痕性脱发，由于不可逆的脱发和瘙痒症状，对生活质量产生重大影响，燃烧和疼痛。它们的特征是毛囊干细胞（HFSCs）的永久丧失，其病理机制仍然知之甚少。导致目前可用的治疗效果不佳。Caveolae是在多种细胞类型的质膜内内陷的烧瓶状脂质筏。尽管它们在HF生理学和病理生理学中的作用相对未知，我们以前已经证明，小窝的主要结构成分（小窝-1或Cav1）在FFA中被上调。因此，我们建议研究LPP和FFA中Caveolae相关结构蛋白(Cav1,Cav2和Cavin-1)和HFSCs(由K15鉴定)的表达和定位.我们分析了4例受影响和未受影响（NA）头皮的LPP活检患者，4例FFA患者在受影响的头皮和4例健康对照中进行了活检。与HC和LPP-NA相比，受影响的LPP和FFA头皮显示出Cav1和Cavin-1的水平升高。此外，Cav1、Cav2和Cavin1均表现出与K15的高度共定位，它们的表达似乎呈负相关，支持以下假设：这些蛋白质在LPP/FFA中起重要作用，并且可能在未来的治疗中用作治疗靶标。
