Abstract：
This research aimed to investigate the effect of slow-released angiogenin by silicon micro-needle on angiogenesis in the Choke zone of dorsal multiple-territory perforator flap in rats, as well as its mechanism. Thirty-six adult Sprague-Dawley (SD) rats were randomly divided into control group, model group, and four experimental groups. In model group, slow-release saline through a silicon micro-needle was placed in choke II zone of the flap 7 days before the operation. For rats in four experimental groups, angiogenin was released via micro-needle in the choke I and choke II zones of the cross-zone flap 7 days before and 3 days before flap surgery, respectively. A 12 cm × 3 cm cross-zone perforator flap model was made on the back of all five groups. The flap survival rate in slow-release angiopoietin group was statistically higher than that in model group (P<0.05). Angiogenin in choke zone of the flap was increased in slow-release angiogenin group (P<0.05). In slow-release angiogenin group, the micro-vessel density was increased and the arteriovenous diameter was decreased, while the arteriovenous diameter was increased in model group (P<0.05). The levels of vascular endothelial growth factor A (VEGF-A) and angiotensin 1 (ANG-1) in choke zone were both elevated in slow-release angiogenin group (P<0.05). The expression of CD31 was significantly elevated in flaps of experimental groups (P<0.05). Micro-needle to slow release Angiogenin can increase the drug concentration in the tissues of the choke zone, promote the vascularization of rat dorsal crossover area perforator flap, reduce the possibility of flap ischemic necrosis, and improve the flap survival rate.
摘要：
本研究旨在研究硅微针缓释血管生成素对大鼠背侧多区域穿支皮瓣窒息区血管生成的影响。以及它的机制。成年SD大鼠36只,随机分为对照组,模型组,和四个实验组。在模型组中，手术前7天，通过硅微针将缓释盐水置于皮瓣的扼流圈II区。对于四个实验组的大鼠，血管生成素在皮瓣手术前7天和前3天通过微针在跨区皮瓣的扼流圈I和扼流圈II区释放，分别。在所有五组的背部制作了12cm×3cm的跨区穿支皮瓣模型。血管生成素缓释组皮瓣成活率高于模型组(P<0.05)。缓释血管生成素组皮瓣阻塞区血管生成素升高(P<0.05)。在缓释血管生成素组,微血管密度增加，动静脉直径减小，模型组动静脉内径增加(P<0.05)。血管生成素缓释组窒息区血管内皮生长因子A(VEGF-A)和血管紧张素1(ANG-1)水平均升高(P<0.05)。实验组皮瓣中CD31的表达明显升高(P<0.05)。微针缓释血管生成素可以增加阻塞区组织中的药物浓度，促进大鼠背侧交叉区域穿支皮瓣的血管化，减少皮瓣缺血性坏死的可能性，提高皮瓣成活率。
