PDF(Pubmed)
Abstract：
Natural and synthetic polymeric materials, particularly soft and hard tissue replacements, are paramount in medicine. We prepared calcium-incorporated sulfonated polyether-ether ketone (SPEEK) polymer membranes for bone applications. The bioactivity was higher after 21 days of immersion in simulated body fluid (SBF) due to calcium concentration in the membrane. We present a new biomaterial healing system composed of calcium and sulfonated polyether ether ketone (Ca-SPEEK) that can function as a successful biomaterial without causing inflammation when tested on bone marrow cells. The Ca-SPEEK exhibited 13 ± 0.5% clot with low fibrin mesh formation compared to 21 ± 0.5% in SPEEK. In addition, the Ca-SPEEK showed higher protein adsorption than SPEEK membranes. As an inflammatory response, IL-1 and TNF-α in the case of Ca-SPEEK were lower than those for SPEEK. We found an early regulation of IL-10 in the case of Ca-SPEEK at 6 h, which may be attributed to the down-regulation of the inflammatory markers IL-1 and TNF-α. These results evidence the innovative bioactivity of Ca-SPEEK with low inflammatory response, opening venues for bone applications.
摘要：
天然和合成聚合材料，特别是软组织和硬组织的替代物，在医学上是最重要的。我们制备了用于骨骼应用的掺入钙的磺化聚醚醚酮（SPEEK）聚合物膜。由于膜中的钙浓度，在模拟体液（SBF）中浸入21天后，生物活性更高。我们提出了一种新的生物材料愈合系统，该系统由钙和磺化聚醚醚酮（Ca-SPEEK）组成，在骨髓细胞上进行测试时，可以作为成功的生物材料发挥作用而不会引起炎症。与SPEEK中的21±0.5%相比,Ca-SPEEK表现出13±0.5%的凝块和低纤维蛋白网眼形成。此外,Ca-SPEEK膜比SPEEK膜显示更高的蛋白质吸附。作为一种炎症反应，Ca-SPEEK的IL-1和TNF-α低于SPEEK。在Ca-SPEEK的情况下，我们发现IL-10在6小时的早期调节，这可能归因于炎症标志物IL-1和TNF-的下调。这些结果证明了Ca-SPEEK具有低炎症反应的创新生物活性，骨应用的开放场所。
