Abstract：
The CLE (CLAVATA3/Embryo Surrounding Region-related) family, a group of peptides with hormone-like features, plays a pivotal role in plant growth, development, and adaptation to stress. Through homology-based blast analysis of 32 Arabidopsis thaliana CLE peptide sequences, we have identified 5, 14, and 10 CLE family members in Nicotiana tabacum, Capsicum annuum, and Solanum melongena, respectively. Chemical synthesis and functional assays of the peptides led to the discovery that NtCLE3 substantially enhances the drought resistance of these three Solanaceae crops. Our transcriptome, RT-qPCR, and antioxidant enzyme activity data showed that NtCLE3 increased antioxidant capacity and ABA synthesis in tobacco. Moreover, the recombinant protein RPNtCLE3, composed of 6*NtCLE3, preserved the capacity to foster drought resilience and proved to be a promising drought resistance regulator, which presents a more favorable alternative for field applications compared to ABA which degrades rapidly under sunlight exposure. This research unveils the prospective utility of NtCLE3 in enhancing drought tolerance in Solanaceae crops and provides new ideas for the development of novel bioregulators aimed at mitigating drought stress.
摘要：
CLE（CLAVATA3/胚胎周围区域相关）家族，一组具有激素样特征的肽，在植物生长中起着举足轻重的作用，发展,适应压力。通过对32个拟南芥CLE肽序列的同源性分析，我们在烟草中确定了5、14和10个CLE家族成员，辣椒，和茄子，分别。肽的化学合成和功能测定导致发现NtCLE3实质上增强了这三种茄科作物的抗旱性。我们的转录组，RT-qPCR,和抗氧化酶活性数据表明，NtCLE3增加了烟草的抗氧化能力和ABA合成。此外，由6*NtCLE3组成的重组蛋白RPNtCLE3保留了增强抗旱性的能力，并被证明是一种有前途的抗旱调节剂，与在阳光照射下快速降解的ABA相比，它为现场应用提供了更有利的替代方案。这项研究揭示了NtCLE3在提高茄科作物耐旱性方面的前景，并为开发旨在缓解干旱胁迫的新型生物调节剂提供了新思路。
