Abstract：
Cell polarity is important for controlling cell shape, motility and cell division processes. Vimentin intermediate filaments are important for cell migration and cell polarization in mesenchymal cells and assembly of vimentin and microtubule networks is dynamically coordinated, but the precise details of how vimentin mediates cell polarity remain unclear. Here, we characterize the effects of vimentin on the structure and function of the centrosome and the stability of microtubule filaments in wild-type and vimentin-null mouse embryonic fibroblasts. We find that vimentin mediates the structure of the pericentriolar material, promotes centrosome-mediated microtubule regrowth and increases the level of stable acetylated microtubules in the cell. Loss of vimentin also impairs centrosome repositioning during cell polarization and migration processes that occur during wound closure. Our results suggest that vimentin modulates centrosome structure and function as well as microtubule network stability, which has important implications for how cells establish proper cell polarization and persistent migration.
摘要：
细胞极性对于控制细胞形状很重要，运动性和细胞分裂过程。波形蛋白中间丝对于间充质细胞中的细胞迁移和细胞极化很重要，波形蛋白和微管网络的组装是动态协调的，但波形蛋白如何介导细胞极性的确切细节仍不清楚。这里,我们描述了波形蛋白对野生型和无波形蛋白小鼠胚胎成纤维细胞中心体的结构和功能以及微管丝稳定性的影响。我们发现波形蛋白介导了中心周围物质的结构，促进中心体介导的微管再生长，并增加细胞中稳定的乙酰化微管的水平。波形蛋白的损失还损害了在伤口闭合期间发生的细胞极化和迁移过程期间的中心体重新定位。我们的结果表明波形蛋白调节中心体结构和功能以及微管网络的稳定性，这对细胞如何建立适当的细胞极化和持续迁移具有重要意义。
